Parasitology Research

, Volume 84, Issue 7, pp 544–548

Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs

  • Monika Zahler
  • Eberhard Schein
  • Heinz Rinder
  • Rainer Gothe
ORIGINAL PAPER

DOI: 10.1007/s004360050445

Cite this article as:
Zahler, M., Schein, E., Rinder, H. et al. Parasitol Res (1998) 84: 544. doi:10.1007/s004360050445

Abstract

The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene were characterized in eight Babesia canis isolates of differing geographic origin, vector specificity, and pathogenicity to dogs. The genotypes determined by sequencing segregated into three clearly separated groups close to or near the species level and correspond to the previously proposed subspecies B. canis canis, B. canis vogeli, and B. canis rossi. The three genotypes can be distinguished by Sau96I digestion of the polymerase chain reaction (PCR)-amplified rDNA target.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Monika Zahler
    • 1
  • Eberhard Schein
    • 2
  • Heinz Rinder
    • 3
  • Rainer Gothe
    • 1
  1. 1.Institut für Vergleichende Tropenmedizin und Parasitologie, Leopoldstrasse 5, D-80802 München, Germany Fax: +49-89-2180-3623; e-mail: monika.zahler@lrz.uni-muenchen.deDE
  2. 2.Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin, Königsweg 67, D-14163 Berlin, GermanyDE
  3. 3.Abteilung für Infektions- und Tropenmedizin, Klinikum Innenstadt der LMU, Leopoldstrasse 5, D-80802 München, GermanyDE

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