Parasitology Research

, Volume 84, Issue 5, pp 399–402 | Cite as

Ultrastructural effects of the chelating agent 1,10-phenanthroline on Trypanosoma cruzi epimastigotes in vitro

  • J. E. Lane
  • B. J. Bogitsh
  • R. Ribeiro-Rodrigues
  • M. V. Kral
  • M. M. Jones
  • C. E. Carter
ORIGINAL PAPER

Abstract

The in vitro effects of the metal chelator 1,10-phenanthroline (OPHEN) on the ultrastructure of Trypanosoma cruzi epimastigotes were investigated. Epimastigotes treated with OPHEN display swelling and electron-dense deposits in the kinetoplast, mitochondrion, and cisternae of the endoplasmic reticulum. These morphological alterations are dose-dependent and first appear at an OPHEN concentration of 5.0 μg/ml. Analytical electron microscope examination indicates that the metallic portion of the electron-dense deposits is predominantly calcium.

Keywords

Calcium Electron Microscope Endoplasmic Reticulum Microscope Examination Analytical Electron Microscope 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. E. Lane
    • 1
  • B. J. Bogitsh
    • 1
  • R. Ribeiro-Rodrigues
    • 1
  • M. V. Kral
    • 2
  • M. M. Jones
    • 3
  • C. E. Carter
    • 1
  1. 1.Department of Biology, Vanderbilt University, Nashville, TN 37235, USAUS
  2. 2.Department of Applied and Engineering Sciences, School of Engineering, Vanderbilt University, Nashville, TN 37235, USAUS
  3. 3.Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USAUS

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