Parasitology Research

, Volume 84, Issue 1, pp 38–40 | Cite as

Molecular cloning and expression of an anti-idiotype antibody mimicking a protective oligosaccharide of the parasite Schistosoma mansoni

  • Karine Petitprez
  • Jean-Marie Grzych
  • Christine Pierrot
  • Claude Godin
  • André Capron
  • Jamal Khalife
ORIGINAL PAPER

Abstract

Genes encoding the heavy and light chains of an anti-idiotype antibody (AB2) mimicking a protective oligosaccharide of Schistosoma mansoni were cloned and expressed as a single-chain Fv fragment. The expression in a functional state was tested using the AB1. A specific binding between sFv and AB1 was observed. Immunization with the recombinant AB2 indicates its capacity to elicit anti-S. mansoni antibodies.

Keywords

Oligosaccharide Functional State Light Chain Specific Binding Molecular Cloning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Karine Petitprez
    • 1
  • Jean-Marie Grzych
    • 1
  • Christine Pierrot
    • 1
  • Claude Godin
    • 1
  • André Capron
    • 1
  • Jamal Khalife
    • 1
  1. 1.INSERM U167 Centre d'Immunologie et de Biologie Parasitaire, Institut Pasteur, F-59019 Lille, France Tel.: (33) 20.87.78.09; Fax: (33) 20.87.78.88; e-mail: karine.petitprez@pasteur-lille.frFR

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