Parasitology Research

, Volume 84, Issue 1, pp 31–37 | Cite as

18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: Ixodidae)

  • A. J. Mangold
  • M. D. Bargues
  • S. Mas-Coma
ORIGINAL PAPER

Abstract

The complete 18S rRNA gene sequences of the following six European hard-tick species were obtained by direct PCR cycle sequencing and silver-staining methods: Rhipicephalus pusillus, Boophilus annulatus, Dermacentor marginatus, Hyalomma lusitanicum, Haemaphysalis punctata, and Ixodes ricinus. Differences observed in the sequence alignment of these six species together with the 18S rRNA gene sequences of 13 other hard-tick species demonstrate that this gene is a good marker for supraspecific differentiation as well as genus grouping among hard ticks. Phylogenetic analyses strongly support that Hyalomma species share a common ancestor with Rhipicephalinae and, consequently, Hyalomminae should no longer be considered an independent subfamily. However, no definitive conclusion could be reached to support or oppose the separation of the subfamilies Haemaphysalinae and Amblyomminae.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • A. J. Mangold
    • 1
  • M. D. Bargues
    • 1
  • S. Mas-Coma
    • 1
  1. 1.Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, E-46100 Burjassot-Valencia, Spain Fax: 34-6-386-47-69 e-mail: S.Mas.Coma@uv.esES

Personalised recommendations