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Parasitology Research

, Volume 83, Issue 7, pp 655–658 | Cite as

Strongyloides stercoralis: identification of antigens in natural human infections from endemic areas of the United States

  • Afzal A. Siddiqui
  • Nora M. Koenig
  • Michael Sinensky
  • Steven L. Berk
ORIGINAL PAPER

Abstract

Using Western-blot analysis, we identified eight immunodominant antigens (apparent molecular weights 96, 86, 75, 56, 41, 32, 28, and 26 kDa) of Strongyloides stercoralis in natural human infections. For this study, 78 individual serum samples were obtained from S.␣stercoralis-infected patients residing in endemic areas of the United States. Poly A+ RNA was translated in vitro in the rabbit-reticulocyte lysate system. The newly synthesized translation products were immuno-precipitated with S. stercoralis human infection sera. All eight of the identified antigens were detected in the immunoprecipitates. The potential of these antigens as targets for immunodiagnosis is also discussed.

Keywords

Molecular Weight Serum Sample Endemic Area Human Infection Apparent Molecular Weight 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Afzal A. Siddiqui
    • 1
  • Nora M. Koenig
    • 1
  • Michael Sinensky
    • 1
  • Steven L. Berk
    • 2
  1. 1.Department of Biochemistry and Molecular Biology, Box 70581, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA Tel: (423) 439-8347/439-8353; Fax: (423) 439-8366; e-mail: siddiqui@access.etsu-tn.eduUS
  2. 2.Department of Internal Medicine James H. Quillen College of Medicine East Tennessee State University Johnson City, TN, USAUS

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