Parasitology Research

, Volume 83, Issue 3, pp 214–219 | Cite as

Characterization of a Swedish bovine isolate of Neospora caninum

  • S. Stenlund
  • C. Björkman
  • O. J. M. Holmdahl
  • H. Kindahl
  • A. Uggla
ORIGINAL PAPER

Abstract

  The brain of a stillborn calf, seropositive to Neospora caninum and born to a seropositive cow, was homogenized and cultured on Vero cells, where growth of Neospora-like tachyzoites was detected after 8 weeks. The ultrastructural features of the new isolate (Nc-SweB1) corresponded to those of previously published Neospora isolates. In indirect immunofluorescence tests, antigens on Nc-SweB1 tachyzoites were recognized by antibodies raised to a canine N. caninum isolate (Nc-1) but not by antibodies to Toxoplasma gondii, Sarcocystis cruzi, S. tenella, Eimeria alabamensis, Babesia divergens, or B. motasi. Immunoblot analyses revealed no major antigenic difference between Nc-SweB1 and Nc-1, whereas several differences were seen between Nc-SweB1 and protozoa related to N. caninum. The sequences of 16S-like rRNA and the internal transcribed spacer 1 of Nc-SweB1 revealed complete homology with corresponding sequences of two canine N. caninum isolates. Thus, no dissimilarity between Nc-SweB1 and the canine isolates was found, confirming that Nc-SweB1 is N. caninum and suggesting that Neospora-like organisms isolated from cattle are indeed N. caninum.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • S. Stenlund
    • 1
  • C. Björkman
    • 3
  • O. J. M. Holmdahl
    • 1
  • H. Kindahl
    • 2
  • A. Uggla
    • 1
  1. 1.Department of Parasitology, National Veterinary Institute and Swedish University of Agricultural Sciences, Box 7073, S-75007 Uppsala, Sweden Fax: + 46 - 18 30 91 62; e-mail: arvid.uggla@sva.seSE
  2. 2.Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Box 7039, S-75007 Uppsala, SwedenSE
  3. 3.Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences, Box 7019, S-75007 Uppsala, SwedenSE

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