Parasitology Research

, Volume 83, Issue 3, pp 214–219 | Cite as

Characterization of a Swedish bovine isolate of Neospora caninum

  • S. Stenlund
  • C. Björkman
  • O. J. M. Holmdahl
  • H. Kindahl
  • A. Uggla
ORIGINAL PAPER

Abstract

  The brain of a stillborn calf, seropositive to Neospora caninum and born to a seropositive cow, was homogenized and cultured on Vero cells, where growth of Neospora-like tachyzoites was detected after 8 weeks. The ultrastructural features of the new isolate (Nc-SweB1) corresponded to those of previously published Neospora isolates. In indirect immunofluorescence tests, antigens on Nc-SweB1 tachyzoites were recognized by antibodies raised to a canine N. caninum isolate (Nc-1) but not by antibodies to Toxoplasma gondii, Sarcocystis cruzi, S. tenella, Eimeria alabamensis, Babesia divergens, or B. motasi. Immunoblot analyses revealed no major antigenic difference between Nc-SweB1 and Nc-1, whereas several differences were seen between Nc-SweB1 and protozoa related to N. caninum. The sequences of 16S-like rRNA and the internal transcribed spacer 1 of Nc-SweB1 revealed complete homology with corresponding sequences of two canine N. caninum isolates. Thus, no dissimilarity between Nc-SweB1 and the canine isolates was found, confirming that Nc-SweB1 is N. caninum and suggesting that Neospora-like organisms isolated from cattle are indeed N. caninum.

Keywords

Immunoblot Analysis Vero Cell Indirect Immunofluorescence Ultrastructural Feature Babesia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • S. Stenlund
    • 1
  • C. Björkman
    • 3
  • O. J. M. Holmdahl
    • 1
  • H. Kindahl
    • 2
  • A. Uggla
    • 1
  1. 1.Department of Parasitology, National Veterinary Institute and Swedish University of Agricultural Sciences, Box 7073, S-75007 Uppsala, Sweden Fax: + 46 - 18 30 91 62; e-mail: arvid.uggla@sva.seSE
  2. 2.Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Box 7039, S-75007 Uppsala, SwedenSE
  3. 3.Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences, Box 7019, S-75007 Uppsala, SwedenSE

Personalised recommendations