Parasitology Research

, Volume 82, Issue 5, pp 439–444

Observations on the ultrastructure and viability of the cystic stage of Blastocystis hominis from human feces

  • K. T. Moe
  • M. Singh
  • J. Howe
  • L. C. Ho
  • S. W. Tan
  • G. C. Ng
  • X. Q. Chen
  • E. H. Yap
Original Paper

Abstract

This report describes the ultrastructure and viability of cysts of Blastocystis hominis from feces of infected patients. The cysts were round to ovoid, measured 2-5 μm in size, and contained a condensed cytoplasm that had vacuoles of varying sizes, four nuclei, and as many as six cristate mitochondria. The cell wall was rather electron-lucent. Surprisingly, chromatoid-like structures were found in the cytoplasm and nucleus of some of the cysts. These have not previously been reported in Blastocystis. The cysts can survive in water for up to 19 days at normal temperatures but are fragile at extreme temperatures and in common disinfectants.

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References

  1. Cassidy MF, Stenzel DJ, Boreham PFL (1994) Electron microscopy of surface structure of Blastocystis sp. from different hosts. Parasitol Res 80:505–511PubMedCrossRefGoogle Scholar
  2. Lee JJ, Hutner SH, Bovee EC (1985) An illustrated guide to the Protozoa. Society of Protozoologists KansasGoogle Scholar
  3. Mehlhorn H (1988) Blastocystis hominis, Brumpt 1912: are there different stages or species? Parasitol Res 74:393–395PubMedCrossRefGoogle Scholar
  4. Silard R, Burghelea B (1985) Ultrastructural aspects of Blastocystis hominis strain resistant to antiprotozoal drugs. Arch Roum Pathol Exp Microbiol 44:73–85PubMedGoogle Scholar
  5. Singh M, Suresh K, Ho LC, Ng GC, Yap EH (1995) Elucidation of the life cycle of the intestinal protozoan Blastocystis hominis. Parasitol Res 81:446–450PubMedCrossRefGoogle Scholar
  6. Stenzel DJ, Boreham PFL (1991) A cyst-like stage of Blastocystis hominis. Int J Parasitol 21:613–615PubMedCrossRefGoogle Scholar
  7. Stenzel DJ, Boreham PFL (1994) Bacteria-like endosymbionts in Blastocystis sp. Int J Parasitol 24:147–149PubMedCrossRefGoogle Scholar
  8. Stenzel DJ, Boreham PFL, McDougall R (1991) Ultrastructure of Blastocvstis hominis in human stool samples. Int J Parasitol 21:807–812PubMedCrossRefGoogle Scholar
  9. Stenzel DJ, Cassidy MF, Boreham PFL (1993) Morphology of Blastocystis sp. from circus animals. Int J Parasitol 23:685–687PubMedCrossRefGoogle Scholar
  10. Stenzel DJ, Cassidy MF, Boreham PFL (1994) Morphology of Blastocystis sp. from domestic birds. Parasitol Res 80:131–137PubMedCrossRefGoogle Scholar
  11. Suresh K, Ng GC, Ramachandran NP, Ho LC, Yap EH, Singh M (1993) In vitro encystment and experimental infections of Blastocystis hominis. Parasitol Res 79:456–460PubMedCrossRefGoogle Scholar
  12. Suresh K, Howe J, Chong SY, Ng GC, Ho LC, Loh AK, Ramachandran NP, Yap EH, Singh M (1994) Ultrastructural changes during in vitro encystment of Blastocystis hominis. Parasitol Res 80:327–335PubMedCrossRefGoogle Scholar
  13. Upcroft JA, Dunn LA, Dommett LS, Healey A, Upcroft P, Boreham PFL (1989) Chromosomes of Blastocystis hominis. Int J Parasitol 19:879–883PubMedCrossRefGoogle Scholar
  14. Zaman V, Khan KH (1994) A concentration technique for obtaining viable cysts of Blastocystis hominis from feces. J Pak Med Assoc 44:220–221PubMedGoogle Scholar
  15. Zaman V, Ng GC, Suresh K, Yap EH, Singh M (1993) Isolation of Blastocystis from the cockroach (Dictyoptera: Blattidae). Parasitol Res 79:73–74CrossRefGoogle Scholar
  16. Zaman V, Howe J, Ng ML (1996) Ultrastructure of Blastocystis hominis cyst. Parasitol Res (in press)Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. T. Moe
    • 1
  • M. Singh
    • 1
  • J. Howe
    • 1
  • L. C. Ho
    • 1
  • S. W. Tan
    • 1
  • G. C. Ng
    • 1
  • X. Q. Chen
    • 1
  • E. H. Yap
    • 1
  1. 1.Department of Microbiology, Faculty of MedicineNational University of SingaporeSingaporeRepublic of Singapore

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