Parasitology Research

, Volume 82, Issue 3, pp 257–260

Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas’ disease described by Carlos Chagas in 1909

  • M. de Lana
  • Clea A. Chiari
  • Egler Chiari
  • Carlos M. Morel
  • Antônio M. Gonçalves
  • Álvaro J. Romanha
ORIGINAL PAPER

DOI: 10.1007/s004360050106

Cite this article as:
de Lana, M., Chiari, C., Chiari, E. et al. Parasitol Res (1996) 82: 257. doi:10.1007/s004360050106

Abstract

 Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas’ disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (k-DNA) minicircles (schizodeme) and isoenzyme patterns (zymodeme). Clear differences were found between the isolates, suggesting the occurrence of a heterogeneous population of T. cruzi in the infection of this patient.

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • M. de Lana
    • 1
  • Clea A. Chiari
    • 2
  • Egler Chiari
    • 2
  • Carlos M. Morel
    • 3
  • Antônio M. Gonçalves
    • 3
  • Álvaro J. Romanha
    • 4
  1. 1.Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Rua Costa Sena, 171, Centro, 35400, MG, Brasil
  2. 2.Departamento de Parasitologia, Instituto de Ciências Biológicas, C.P. 2486, Universidade Federal de Minas Gerais, 31270, Belo Horizonte, MG, Brasil
  3. 3.Departamento de Bioquímica e Biologia Molecular, IOC-FIOCRUZ, Rio de janeiro, RJ, Brasil
  4. 4.Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, Brasil

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