Abstract
Schistosomiasis remains a parasitic infection which poses serious public health consequences around the world, particularly on the African continent where cases of introgression/hybridization between human and cattle schistosomiasis are being discovered on a more frequent basis in humans, specifically between Schistosoma haematobium and S. bovis. The aim of this paper is to analyze the occurrence of S. bovis in cattle and its relationship with S. haematobium in an area where cattle and humans share the same site in Benin (West Africa). We used the chronobiology of cercarial emergence as an ecological parameter and both molecular biology (COI mtDNA and ITS rDNA) of the larvae and morphology of the eggs as taxonomic parameters. The results showed a chronobiological polymorphism in the cercarial emergence rhythm. They showed for the first time the presence of S. bovis in Benin, the presence of introgressive hybridization between S. bovis and S. haematobium in domestic cattle, and the presence of atypical chronobiological patterns in schistosomes from cattle, with typical S. haematobium shedding pattern, double-peak patterns, and nocturnal patterns. Our results showed that the chronobiological life-history trait is useful for the detection of new hosts and also may reveal the possible presence of introgressive hybridization in schistosomes. Our results, for the first time, place cattle as reservoir host for S. haematobium and S. bovis x S. haematobium. The consequences of these results on the epidemiology of the disease, the transmission to humans, and the control of the disease are very important.
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Acknowledgments
We would like to thank M. Marius CODJO, chief of Kessounou village and all the breeders and also M. Jean-Pierre TOKPO, Director of Kessounou school and all the schoolchildren for their participation to the study.
Funding
This research was funded by the French Ministry of Foreign Affairs, the “Laboratoire Mixte International” (Mixed International Laboratory) on the project CONS-HELM « Infections helminthiques: traitements et conséquences sur la santé et le développement au Sud », and the French National Agency for Research (ANR) [grant ANR-17-CE12-0005-01] CHRONOGET and CNRS. BS is a student fellow from the SCAC (Service de Coopération et d’Action Culturelle), French Embassy in Benin; CL is a student fellow from the Occitanie Region, France.
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Contributions
Conceptualization: Hélène Moné, Gabriel Mouahid, Moudachirou Ibikounlé.
Material preparation, data collection and analysis: Boris A.E.S. Savassi, Gabriel Mouahid, Chrystelle Lasica, Samoussou-Dine K. Mahaman, Jean-François Allienne, Moudachirou Ibikounlé, Hélène Moné.
Funding acquisition: Gabriel Mouahid, André Garcia, David Courtin, Moudachirou Ibikounlé, Hélène Moné.
Investigation: Boris A.E.S. Savassi, Gabriel Mouahid, Chrystelle Lasica, Samoussou-Dine K. Mahaman, Jean-François Allienne, Moudachirou Ibikounlé, Hélène Moné.
Methodology: Hélène Moné, Gabriel Mouahid, Moudachirou Ibikounlé.
Project administration: Gabriel Mouahid, André Garcia, David Courtin, Moudachirou Ibikounlé, Hélène Moné.
Resources: Boris A.E.S. Savassi, Gabriel Mouahid, Chrystelle Lasica, Samoussou-Dine K. Mahaman, André Garcia, David Courtin, Jean-François Allienne, Moudachirou Ibikounlé, Hélène Moné.
Supervision: Gabriel Mouahid, Hélène Moné.
Validation: Gabriel Mouahid, Hélène Moné.
Visualization: Gabriel Mouahid, Hélène Moné.
Writing–original draft: Boris A.E.S. Savassi, Gabriel Mouahid, Hélène Moné.
Writing–review & editing: Boris A.E.S. Savassi, Gabriel Mouahid, Chrystelle Lasica, André Garcia, David Courtin, Jean-François Allienne, Moudachirou Ibikounlé, Hélène Moné.
All authors read and approved the final manuscript.
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The authors declare that they have no conflict of interest.
Ethical approval
Ethical permission (No119, 17/07/2019/CER-ISBA) was obtained from the Ethic Committee of the « Institut des Sciences Biomédicales Appliquées, Faculté de Médecine, Université d’Abomey-Calavi, 01BP526, Cotonou, Benin.
Informed consent
The Director of Kessounou school obtained informed consent from the parents of all the schoolchildren who participated to the study.
Data set availability
Sequence data were deposited in the NCBI GenBank database under the accession numbers MT159589 to MT159601 for COI mtDNA and MT158872 to MT158882 for ITS rDNA.
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Savassi, B.A., Mouahid, G., Lasica, C. et al. Cattle as natural host for Schistosoma haematobium (Bilharz, 1852) Weinland, 1858 x Schistosoma bovis Sonsino, 1876 interactions, with new cercarial emergence and genetic patterns. Parasitol Res 119, 2189–2205 (2020). https://doi.org/10.1007/s00436-020-06709-0
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DOI: https://doi.org/10.1007/s00436-020-06709-0