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Atypical Toxoplasma gondii genotype from a sheep and a pig on Fernando de Noronha Island, Brazil, showed different mouse virulence profiles

  • Renata Pimentel B. MeloEmail author
  • Jonatas C. Almeida
  • Débora C. V. de Lima
  • Jéssica C. S. Carvalho
  • Wagnner J. N. Porto
  • Fernando J. R. Magalhães
  • Clare M. Hamilton
  • Frank Katzer
  • Rinaldo A. Mota
Protozoology - Short Communication

Abstract

Toxoplasma gondii is a zoonotic parasite which can infect almost all warm-blooded animals. Toxoplasma gondii isolates from Brazil have greater genetic diversity with a predominance of virulent and atypical genotypes, compared with the Northern Hemisphere. Considering that previous studies have demonstrated a high seroprevalence of T. gondii antibodies in animals from Fernando de Noronha Island, the aim of this study was to isolate, genetically characterize, and determine mouse virulence of isolates of T. gondii from livestock from this Brazilian island. Two T. gondii isolates were obtained by mouse bioassay from brain from one sheep and one pig. Genotyping was performed by PCR-RFLP using 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22- 8, c29-2, PK1, L358, and Apico) and an atypical genotype of T. gondii (ToxoDB #146) was identified for both isolates. Genotyping of four ROP loci indicated different alleles for ROP16 and mouse virulence analysis revealed different profiles (intermediate and low virulence). This is the first report of this genotype being described in a pig and a sheep.

Keywords

Mouse bioassay Isolation Genotyping Virulence genes 

Notes

Acknowledgments

The authors thank the Health Superintendence of Fernando de Noronha Island for all the support during the sample collection.

Funding information

This study was supported by Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE, APQ-0531-5.05/14). We would like to thank to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, for the scholarship (SWE, grant number 206740/2017-4) and to FACEPE for the PhD scholarship (IBPG-1358-5.05./15). The authors thank the Health Superintendence of Fernando de Noronha Island for all the support during the sample collection.

Compliance with ethical standards

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. The study was approved by the Ethics Committee in Animal Use at Universidade Federal Rural de Pernambuco (UFRPE) (license number 139/2016).

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Renata Pimentel B. Melo
    • 1
    Email author
  • Jonatas C. Almeida
    • 1
  • Débora C. V. de Lima
    • 1
  • Jéssica C. S. Carvalho
    • 1
  • Wagnner J. N. Porto
    • 2
  • Fernando J. R. Magalhães
    • 3
  • Clare M. Hamilton
    • 4
  • Frank Katzer
    • 4
  • Rinaldo A. Mota
    • 1
  1. 1.Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic AnimalsUniversidade Federal Rural de PernambucoRecifeBrazil
  2. 2.Biological and Health Science InstituteUniversidade Federal de AlagoasMaceióBrazil
  3. 3.Health SuperintendenceAdministração do Distrito Estadual de Fernando de NoronhaPernambucoBrazil
  4. 4.Moredun Research InstituteScotlandUK

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