Sperm characters of the aspidogastrean Rohdella amazonica (Aspidogastridae, Rohdellinae), a parasite of the banded puffer fish Colomesus psittacus

  • Elane G. Giese
  • Raul Henrique S. Pinheiro
  • Zdzisław Świderski
  • Jordi MiquelEmail author
Helminthology - Original Paper


The ultrastructural characteristics of the mature spermatozoon of the aspidogastrean Rohdella amazonica (Aspidogastridae, Rohdellinae) were studied by means of transmission electron microscopy. The sperm cell shows two axonemes of the 9 + ‘1’ trepaxonematan pattern of Platyhelminthes, parallel cortical microtubules, a well-developed lateral expansion, external ornamentation of the plasma membrane, one mitochondrion, an electron-dense ring, a nucleus and granules of glycogen. The present results were compared with those observed in the aspidogastreans studied to date and in other Platyhelminthes. The lateral expansion and the electron-dense ring are typical characters for aspidogastreans. Although a lateral expansion has been described in other Platyhelminthes such as monogeneans and digeneans, the Aspidogastrea shows a much larger lateral expansion with both peripheral and internal microtubules. The dense ring is observed as a cylinder in a longitudinal view and shows a more granular appearance in sperm cells from the seminal vesicle in comparison to a more electron-dense appearance in sperm cells from the seminal uterine receptacle.


Rohdella amazonica Aspidogastrea Aspidogastridae Rohdellinae Sperm characters Ultrastructure 



The authors wish to thank the personnel of the ‘Unitat de Microscòpia Electrònica, Facultat de Medicina, Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB)’ for their support in the preparation of samples. JM is a member of the 2017-SGR-1008 research group.

Funding information

This work was partly supported by CAPES post-doctoral fellowship to EGG (21/2018 PROCAD AMAZÔNIA-DRI-88881.314463/2019-01) and by ISPA-UFRA.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção AnimalUniversidade Federal Rural da AmazôniaBelémBrazil
  2. 2.Secció de Parasitologia, Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l’AlimentacióBarcelonaSpain
  3. 3.Instituto de Ciências e Tecnologia das ÁguasUniversidade Federal do Oeste do ParáSantarémBrazil
  4. 4.Witold Stefański Institute of ParasitologyPolish Academy of SciencesWarszawaPoland
  5. 5.Institut de Recerca de la Biodiversitat (IRBio)Universitat de BarcelonaBarcelonaSpain

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