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Immunodiagnosis of human neurocysticercosis: comparative performance of serum diagnostic tests in Mexico

  • Marisela Hernández
  • Osvaldo-Germán Astudillo
  • Gustavo Diego
  • Jorge-Luis de-la-Rosa-Arana
  • Antonio Meza-Lucas
  • Ricardo García-Rodea
  • Matthew-L. Romo
  • Andrea Toledo
  • R.-Michael-E. Parkhouse
  • Teresa Garate
  • Edda Sciutto
  • Agnès FleuryEmail author
Helminthology - Original Paper
  • 4 Downloads

Abstract

Immunodiagnosis has a supportive role in the diagnosis of neurocysticercosis (NCC). The aim of this study was to compare the validity of seven immunodiagnostic tests among serum samples from 58 patients with NCC, 26 patients with neurological diseases other than NCC, and 15 healthy controls. One test for viable parasite detection (HP10 antigen assay) and six for antibody detection were evaluated. For the entire sample, sensitivities ranged from 55.2% (NOVALISA) to 81.0% (enzyme-linked immunosorbent assay [ELISA] Taenia solium antibody), with the sensitivity of the latter test significantly higher than that of the in-house ELISA Taenia crassiceps, NOVALISA, enzyme-linked immunoelectrotransfer blot (EITB) CDC, and HP10. Overall, specificities were high, ranging from 85.4% (ELISA Ts) to 97.1% (NOVALISA), with no statistically significant differences. Detection of HP10 antigen was significantly associated with the presence of vesicular parasites. The simple and low-cost ELISA Taenia solium antibody Ab instead of EITB is recommended to support NCC diagnosis in both rural and hospital settings in Mexico.

Keywords

ELISA EITB HP10 Immunodiagnosis Mexico Neurocysticercosis 

Notes

Authors’ contributions

This study was designed, supervised, and coordinated by RMEP, TG, ES, and AF. MH, OGA, GD, JLRA, AML, RGR, and AT carried out the clinical assessment and the immunoassays; MLR and AF carried out the analysis and interpretation of these data. JLRA, RMEP, ES, and AF drafted the manuscript. All authors critically revised the manuscript for intellectual content, read, and approved the final manuscript. ES and AF are guarantors of the paper.

Funding information

This work was partially supported by the “Programa Iberoamericano de Ciencia y TecnologÍa para el Desarrollo, CYTED” [211RT0432] and by the “Programa de Investigación para el Desarrollo y la Optimización de Vacunas, Inmunomoduladores y Métodos Diagnósticos del IIB” (PROVACADI, Instituto de Investigaciones Biomédicas, UNAM, México).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

436_2019_6425_MOESM1_ESM.docx (21 kb)
ESM 1 (DOCX 20 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Marisela Hernández
    • 1
  • Osvaldo-Germán Astudillo
    • 2
  • Gustavo Diego
    • 2
  • Jorge-Luis de-la-Rosa-Arana
    • 3
  • Antonio Meza-Lucas
    • 3
  • Ricardo García-Rodea
    • 3
  • Matthew-L. Romo
    • 4
  • Andrea Toledo
    • 5
  • R.-Michael-E. Parkhouse
    • 6
  • Teresa Garate
    • 7
  • Edda Sciutto
    • 1
  • Agnès Fleury
    • 5
    • 8
    Email author
  1. 1.Departamento de InmunologíaInstituto de Investigaciones Biomédicas, UNAMCiudad de MéxicoMexico
  2. 2.Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) “Dr. Carlos Malbrán”Buenos AiresArgentina
  3. 3.Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE), Secretaria de SaludCiudad de MéxicoMexico
  4. 4.School of Public Health, Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongHong Kong
  5. 5.Unidad de NeuroinflamaciónInstituto de Investigaciones Biomédicas-UNAM/Instituto Nacional de Neurología y Neurocirugía/Facultad de Medicina—UNAMCiudad de MéxicoMexico
  6. 6.Instituto Gulbenkian de Ciência (IGC)LisbonPortugal
  7. 7.Instituto de Salud Carlos IIIMadridSpain
  8. 8.Neurocysticercosis ClinicInstituto Nacional de Neurología y NeurocirugíaCiudad de MéxicoMexico

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