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Parasitology Research

, Volume 118, Issue 6, pp 1953–1961 | Cite as

Synthesis and in vitro activity of new biguanide-containing dendrimers on pathogenic isolates of Acanthamoeba polyphaga and Acanthamoeba griffini

  • T. Martín-PérezEmail author
  • T. Lozano-Cruz
  • A. Criado-Fornelio
  • P. Ortega
  • R. Gómez
  • F. J. de la Mata
  • J. Pérez-Serrano
Treatment and Prophylaxis - Original Paper

Abstract

The genus Acanthamoeba can cause Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). The treatment of these illnesses is hampered by the existence of a resistance stage that many times causes infection relapses. In an attempt to add new agents to our chemotherapeutic arsenal against acanthamebiasis, two Acanthamoeba isolates were treated in vitro with newly synthesized biguanide dendrimers. Trophozoite viability analysis and ultrastructural studies showed that dendrimers prevent encystment by lysing the cellular membrane of the amoeba. Moreover, one of the dendrimers showed low toxicity when tested on mammalian cell cultures, which suggest that it might be eventually used as an amoebicidal drug or as a disinfection compound in contact lens solutions.

Keywords

Acanthamoeba Genotype T3 Genotype T4 Trophozoites Biguanide Dendrimers 

Notes

Acknowledgments

We wish to thank Antonio Priego and Mr. José Antonio Pérez (Microscopy Unit–CAI Medicina y Biología de la Universidad de Alcalá) for assistance with scanning electron microscopy and Ángel Pueblas (Photography Unit–CAI Medicina y Biología de la Universidad de Alcalá) for expert help with photographic work.

Funding information

This work was supported by grants CTQ2017-86224-P (from MINECO), Consortium NANODENDMED-II-CM (B2017/BMD-3703), IMMUNOTHERCAN-CM (B2017/BMD3733), and Universidad de Alcalá CCG2016/BIO-023. CIBER-BBN as an initiative funded by the VI National R&D&i Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions, and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.

Supplementary material

436_2019_6341_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 16 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Biomedicina y Biotecnología, Laboratorio de Parasitología, Grupo ECOMYP, Facultad de FarmaciaUniversidad de AlcaláMadridSpain
  2. 2.Instituto de Investigación Química “Andrés M. del Río” (IQAR), Departamento de Química Orgánica y Química InorgánicaUniversidad de Alcalá, Campus UniversitarioMadridSpain
  3. 3.Networking Research Center on BioengineeringBiomaterials and Nanomedicine (CIBER-BBN)MálagaSpain
  4. 4.Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)MadridSpain

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