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Parasitology Research

, Volume 118, Issue 6, pp 1841–1848 | Cite as

Increased phagocytosis and growth inhibition of Encephalitozoon cuniculi by LPS-activated J774A.1 murine macrophages

  • J. R. González-Machorro
  • L. E. Rodríguez-Tovar
  • R. Gómez-Flores
  • A. Soto-Dominguez
  • H. Rodríguez-Rocha
  • A. Garcia-García
  • P. Tamez-Guerra
  • U. Castillo-VelázquezEmail author
Immunology and Host-Parasite Interactions - Original Paper
  • 36 Downloads

Abstract

Encephalitozoon cuniculi is an obligate macrophage parasite of vertebrates that commonly infects rodents, monkeys, dogs, birds, and humans. In the present study, we aimed to assess the phagocytosis and intracellular survival of E. cuniculi spores using untreated and lipopolysaccharide (LPS)-activated J774A.1 murine macrophages and assess the macrophage viability. The experimental groups comprised untreated spores, spores killed by heat treatment at 90 °C, and spores killed by treatment with 10% formalin. LPS-activated macrophages significantly increased the phagocytosis of spores and reduced their intracellular growth after 24 and 48 h (P < 0.01); however, after 72 h, we observed an increase in spore replication but no detectable microbicidal activity. These results indicate that LPS activation enhanced E. cuniculi phagocytosis between 24 and 48 h of treatment, but the effect was lost after 72 h, enabling parasitic growth. This study contributes to the understanding of the phagocytosis and survival of E. cuniculi in murine macrophages.

Keywords

Encephalitozoon cuniculi Phagocytosis Macrophages Intracellular survival LPS activation Nitric oxide 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • J. R. González-Machorro
    • 1
  • L. E. Rodríguez-Tovar
    • 1
  • R. Gómez-Flores
    • 2
  • A. Soto-Dominguez
    • 3
  • H. Rodríguez-Rocha
    • 3
  • A. Garcia-García
    • 1
  • P. Tamez-Guerra
    • 2
  • U. Castillo-Velázquez
    • 1
    Email author
  1. 1.Facultad de Medicina Veterinaria y Zootecnia, Departamento de Inmunología Veterinaria, Campus de Agricultura y Ciencias BiológicasUniversidad Autónoma de Nuevo LeónEscobedoMexico
  2. 2.Facultad de Ciencias Biológicas, Departamento de Microbiología e InmunologíaUniversidad Autónoma de Nuevo LeónSan Nicolás de los GarzaMexico
  3. 3.Facultad de Medicina, Departamento de HistologíaUniversidad Autónoma de Nuevo LeónMonterreyMexico

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