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Detection and genotypic characterization of Toxoplasma gondii DNA within the milk of Mongolian livestock

  • E. Iacobucci
  • N. S. Taus
  • M. W. Ueti
  • L. Sukhbaatar
  • Z. Bastsukh
  • S. Papageorgiou
  • H. FritzEmail author
Open Access
Protozoology - Short Communication

Abstract

Toxoplasma gondii is a global, zoonotic parasite capable of infecting any warm-blooded host. Toxoplasmosis can cause a variety of illnesses including abortions and congenital defects in humans, sheep, and goats. Congenital toxoplasmosis is considered to have the highest global disease burden of any foodborne illness in humans. This study examined the potential role of milk as a route of T. gondii transmission between livestock and humans within Mongolian herders, a little-studied population which relies heavily on animals. Milk of Mongolian sheep, goats and Bactrian camels was tested for the presence of T. gondii DNA, and a survey was conducted to ascertain what behavioral and environmental factors were present that might potentiate T. gondii infection within these Mongolian communities. T. gondii DNA was detected in samples from one sheep and five camels. Sequence analysis of DNA from camel milk revealed that two were from potentially virulent T. gondii genotypes. This has implications for public health in the region, as milk is an extremely important source of nutrition and our survey results imply that some people believe consumption of raw camel milk carries health benefits. This is the first report of T. gondii DNA in Bactrian camel milk as well as the first genotypic characterization of T. gondii within Mongolia.

Keywords

Toxoplasma gondii Bactrian camel Mongolia Milk PCR 

Introduction

Toxoplasma gondii is a protozoan parasite capable of infecting any warm-blooded host. An estimated one third of the world human population is chronically infected (Weiss and Dubey 2009). T. gondii’s complex life cycle, environmentally resistant oocyst stage, and multiple routes of infection make it a successful and ubiquitous zoonotic pathogen that infects humans, companion and livestock animals, and wildlife species around the world. Few data are available concerning T. gondii in Asia, and almost none are available in Mongolia. The goal of this study was to determine if T. gondii DNA is present in the milk of Mongolian livestock and ascertain what behavioral and environmental factors are present that may potentiate T. gondii infection within this community.

There is conflicting information regarding the risk of human infection with T. gondii via consumption of unpasteurized milk. However, in goat milk, T. gondii has been shown to be viable and in high enough numbers to infect mice and cats, both in raw milk and in cold enzyme–produced cheese products (Dubey et al. 2014). Raw dromedary camel milk has been proven to contain organisms capable of infecting camel calves and mice (Boughattas 2017). Bactrian camels have been found positive for T. gondii by serological tests, but there is no published data currently about detection of T. gondii in their milk (Wang et al. 2013). For Mongolians practicing nomadic pastoralism, toxoplasmosis may currently be an unidentified health threat. The United Nation’s Food and Agricultural Organization reports that annual milk consumption per person in rural Mongolia was 200 kg liquid milk equivalents (LME) compared with only 50 kg LME in Mongolian urban centers in 2005 (with > 150 kg LME considered “high” by global comparison) (Setsgee Ser-Od and Dugdill 2010). Liquid milk and traditional milk products are extremely culturally important and considered sacred in some religious and cultural groups. Despite the population’s dependence on livestock and the intimate relationship between the health of herding families and that of their animals, limited data are published about zoonotic disease in Mongolia.

There are three primary routes of infection, including ingestion of sporulated oocysts in contaminated water or food, ingestion of tissue cysts from undercooked animal products (bradyzoites) and transplacental transmission (tachyzoites) (Tenter et al. 2000, Rico-Torres et al. 2016). Toxoplasma undergoes sexual reproduction only within its definitive felid hosts resulting in production of the environmentally stable oocyst (Dubey et al. 1970; Frenkel et al. 1970). When sporulated oocysts are ingested by a susceptible intermediate host, T. gondii is capable of infecting any nucleated cell type (Dubey et al. 1998; Ferguson and Hutchison 1987; Guimaraes et al. 2008; Sims et al. 1989). As a result of the formation of latent tissue cyst stages following infection, the host remains infected for life (Tenter et al. 2000). T. gondii virulence among the three clonal lineages has been extensively studied by experimental mouse infections, with type I isolates being highly virulent in mice and types II and III less virulent or avirulent, respectively. Although the majority of hosts infected with T. gondii never display overt clinical signs, the infecting genotype may be an important determinant of virulence and clinical outcome of infection. Furthermore, T. gondii infection may pose a major risk to the developing fetus when infected transplacentally and for immunocompromised individuals, irrespective of the infecting genotype. While the importance and extent of T. gondii transmission via milk is not currently known, the demonstration herein of T. gondii DNA in milk and its genotypic characterization are important preliminary steps in directing future research and developing food safety recommendations to herding families.

Materials and methods

Milk samples were collected from 19 herds within two Mongolian provinces, as shown in Fig. 1. Seven sampling sites were in Tuv province and nine in Omnigovi province. No more than 6 healthy animals per species were selected from each herd. A total of 59 Mongolian fuzzy goats, 58 Mongolian fat-tailed sheep, and 9 Bactrian camels were sampled.
Fig. 1

Study sites. Milk samples were collected at sites indicated by black dots. Pink triangles indicate the locations where Toxoplasma was detected in camel milk. The green triangle indicates the location where Toxoplasma was detected in sheep milk

DNA was extracted from 2 ml of milk from each individual animal using a DNeasy Blood & Tissue Kit (Qiagen) with some modifications to the manufacturer’s protocol. Each DNA product was first screened for DNA of the species by which the milk was produced as an internal control to ensure the extraction worked. Primers targeting the conserved gene DQA2 were used to screen for sheep and goat DNA while primers targeting the mitochondrial cytb gene were used to screen for Bactrian camel DNA. Samples which amplified internal control host species DNA were then screened in triplicate by PCR for T. gondii DNA targeting the ITS-1 and B1 genes by nested PCR. All primers are listed in online resource EMS_1. Positive amplicons were cleaned with ExoSAP-IT (ThermoFisher Scientific), sequenced to confirm amplicon specificity (Eurofins MWG Operon, Louisville, KY), and results were compared to published T. gondii genomic sequences in BLAST (blast.ncbi.nlm.nih.gov/Blast.cgi).

A survey regarding herd health, animal product use, and environmental factors was administered to the family head or heads in 16 of the 19 families visited. The survey questions and consent form are online resources EMS_ 2 and EMS_3.

Results and discussion

Toxoplasma gondii DNA was detected in the milk of one sheep in Tuv province and eight Bactrian camels in Omnigovi. Genotyping of the T. gondii detected in four of the camels was done by examining bases numbered 366 and 504 in the published B1 sequence (GenBank accession number AF179871) (Grigg and Boothroyd 2001). At these locations, sequences from two camel samples had double peaks in the chromatogram, indicative of Toxoplasma type II and III lineages (Fig. 2). Chromatograms of T. gondii DNA from two other camel samples show only one nucleotide peak, which is indicative of a type I or atypical lineage (Fig. 2). Although the sample size is small, the results offer a glimpse of an unexpected ecology of T. gondii in the region. The detection of T. gondii in five of eight camels indicates that camels may play an important role in the T. gondii life cycle in the deserts of Asia. There is only one published report of a T. gondii seropositive Bactrian camel. It was located in Qinghai Province, China, approximately 1000 air km from this investigation’s milk positive camels (Wang et al. 2013). To the authors’ knowledge, this is the first report of T. gondii DNA detection within milk of Bactrian camels and the first genotypic characterization of Toxoplasma gondii within Mongolia. The detection of potential type I T. gondii DNA in camel milk is concerning given that it is considered more virulent than types II and III, as reflected by a lower LD50 in experimentally infected mice and their increased association with human congenital toxoplasmosis compared with the other lineages (Sibley and Boothroyd 1992).
Fig. 2

Chromatogram excerpts surrounding XhoI and PmlI sites within the B1 sequences of Toxoplasma gondii DNA extracted from camel milk. The codes on the left represent the individual animal and family. Asterisks are located over the nucleotides of interest which exhibit polymorphisms used to distinguish lineages. In OCa1 and OCa5, there are two nucleotide peaks at both locations. In OCa3 and OCa8, there is a single, strong nucleotide peak at these locations

Four surveys were completed in Terelj, three were completed in Hustai, and nine were completed in Gobi. More than half of the herders interviewed in this study believed domestic cats lived in near vicinity to their current homesteads, rising to over 80% when both domestic and wild cats (Siberian lynx, Pallas cat, and snow leopard) were included. Of the 16 herders interviewed, 56.25% reported a history of goat abortion in their herd, 18.75% reported a history of sheep abortions and one family (6.25%) reported a history of camel abortion. One serologic study examining T. gondii infection among sheep in seven northern provinces in Mongolia found infection in 16.57% by latex agglutination test and 24% by ELISA (Tumurjav et al. 2010). The prevalence of infection was low but ubiquitous in that study. This study’s survey reveals the elements to the T. gondii life cycle are present and the abortions reported could be part of pathologic effects on herd health. In addition to this study’s discovery of a potentially virulent genotype in Bactrian camel milk, one family reported consuming milk raw. The rest explained that the milk was always heated first, usually by simmering or boiling for a minimum of 15–20 min. People were generally aware that milk should be treated before consumption; however, the family reporting raw camel milk use explained their belief that it has health benefits which heated milk did not possess. The raw milk consumption reported in the survey is one possible route of transmission from livestock to humans.

Little data are available on the impact of zoonotic disease on the humans and livestock of Mongolia, including the impact of toxoplasmosis on humans in this country. For Mongolian herders, the risk factors exist for transmission, and according to our results, a potentially dangerous lineage of T. gondii is endemic in the area. Genotypic characterization of additional DNA samples would help to determine the extent to which virulent type I genotypes are circulating in the region. A better understanding of T. gondii biology within Bactrian camels would be extremely interesting, as this is an economically important species in several countries. Herders will benefit from knowledge of potential health threats and could direct efforts to build resilience mechanisms for their communities.

Notes

Acknowledgments

The authors thank the Institute of Veterinary Medicine in UlaanBaatar for use of laboratories for sample processing; staff of the Mongolian Bankhar Dog Project for support with herder networking, transportation, and logistical organization; and Soyolbolod Serguleng for translation and serving as cultural liaison. The authors thank Janelle Sagawa, Gregory Bowden, and Jessica McCrea of Washington State University for technical advice and discussions, and Nathan Roueche of Western Washington University for GIS work.

Funding information

This work was supported by Washington State University College of Veterinary Medicine Summer Research Fellowship and Washington State University Graduate and Professional Student Association Travel Grant (EI), NIH KO1 Award Number KO1RR031487 from the National Center for Research Resources and start-up funds from WSU to HMF.

Compliance with ethical standards

Consent forms were discussed and signed before the animal handling or the survey began. The consent form included a section in which the participant could indicate if they want to know the results of the animal testing and a method of contacting them with that information. The Washington State University IRB considered this survey and determined it exempt from needing a full IRB review.

The Institutional Animal Care and Use Committee at Washington State University approved Animal Subjects Protocol, No. 04693-001, on June 4, 2015.

Supplementary material

436_2019_6306_MOESM1_ESM.docx (13 kb)
ESM 1 Table of primers used in the study (DOCX 13 kb)
436_2019_6306_MOESM2_ESM.pdf (127 kb)
ESM 2 Survey questions asked in the study (PDF 127 kb)
436_2019_6306_MOESM3_ESM.pdf (175 kb)
ESM 3 Consent form for study Detection and genotypic characterization of Toxoplasma gondii DNA within the milk of Mongolian livestock (PDF 175 kb)

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Copyright information

© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.College of Veterinary MedicineWashington State UniversityPullmanUSA
  2. 2.Animal Disease Research Unit, Agricultural Research ServiceU.S. Department of AgriculturePullmanUSA
  3. 3.Laboratory of HelminthologyInstitute of Veterinary Medicine UlaanBaatarUlaanBaatarMongolia
  4. 4.School of Veterinary MedicineUniversity of California DavisDavisUSA

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