Pseudokabatana alburnus n. gen. n. sp., (Microsporidia) from the liver of topmouth culter Culter alburnus (Actinopterygii, Cyprinidae) from China
We describe the type species of a novel genus of microsporidian parasite, Pseudokabatana alburnus n. gen. n. sp., infecting the liver of topmouth culter, Culter alburnus Basilewsky, 1855, from Lake Poyang off Xingzi county, Jiangxi Province, China. The parasite elicits formation of spherical xenomas of up to 1.2 mm in diameter containing all observed life stages from early merogonal plasmodia to mature spores contained within the cytoplasm of host hepatocytes. Merogonal plasmodia existed in direct contact with the host cytoplasm and contained up to 20 visible nuclei. Plasmotomy of the multinucleate plasmodium led to formation of uninucleate cells in which the nucleus underwent further division to form bi-nucleate presporonts, sporonts (defined by cells with a thickened endospore) and eventually sporoblasts (containing pre-cursors of the spore extrusion apparatus). Mature spores were pyriform and monokaryotic, measuring 2.3 ± 0.19 μm long and 1.3 ± 0.10 μm wide. Spores possessed a bipartite polaroplast and 5–6 coils of a polar filament, in a single rank. The obtained partial SSU rRNA gene sequence, 1383 bp in length, did not match any of microsporidia available in GenBank. SSU rDNA-based phylogenetic analysis indicated a new taxon branching with Kabatana rondoni, a parasite infecting the skeletal muscle of Gymnorhamphichthys rondoni from the Amazon River. Due to different host and tissue tropism, the novel taxon did not fit the diagnostic criteria for the genus Kabatana. Further, based on SSU rDNA-inferred phylogenetic analyses, different ultrastructural features of developmental stages, and ecological considerations, a new genus Pseudokabatana and type species Pseudokabatana alburnus n. sp. was erected for the parasite in topmouth culter.
KeywordsKabatana Pseudokabatana alburnus n. gen. n. sp. Ultrastructure Xenoma
The authors are much indebted to Yuliya Y. Sokolova (Department of Comparative Biological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge LA, USA and Institute of Cytology, Russian Academy of Sciences, Russia) and Tokarev YS and Issi IV (All-Russian Institute of Plant Protection, Russian Academy of Agriculture Sciences, Russia) for their help to discern the ultrastructural characters.
The study was financially supported by Natural Science Foundation of China (31772411) and Free-Orientation project of Institute of Hydrobiology, Chinese Academy of Sciences. GDS was supported by the UK department for Environment, Food and Rural Affairs (Defra) under contract FB002.
- Cao WX (2011) The current situation and protection strategies of fish resources in Yangtze River. Jiangxi Fish Sci Technol 2:1–4 (In Chinese)Google Scholar
- Casal G, Rocha S, Costa G, Al-Quraishy S, Azevedo C (2016) Ultrastructural and molecular characterization of Glugea serranus n. sp., a microsporidian infecting the black tail comber, Serranus atricauda (Teleostei: Serranidae), in the Madeira archipelago (Portugal). Parasitol Res 115:3963–3972CrossRefGoogle Scholar
- Chen QL (1955) The protozoan parasites from four species of Chinese pond fishes: Ctenopharyngodon idellus, Mylopharyngodon piceus, Aristichthys nobillis and Hypophthalmichthys molithrix I: the protozoan parasites of Ctenopharyngodon idellus. Acta Hydrobiol Sin 1:123–164 (In Chinese)Google Scholar
- Chen QL (1956a) The protozoan parasites from four species of Chinese pond fishes: Ctenopharyngodon idellus, Mylopharyngodon piceus, Aristichthys nobillis and Hypophthalmichthys molithrix II. The protozoan parasites of Mylopharyngodon piceus. Acta Hydrobiol Sin 1:19–42 (In Chinese)Google Scholar
- Chen QL (1956b) The protozoan parasites from four species of Chinese pond fishes: Ctenopharyngodon idellus, Mylopharyngodon piceus, Aristichthys nobillis and Hypophthalmichthys molithrix III. The protozoan parasites of Aristichthys nobillis and Hypophthalmichthys molithrix. Acta Hydrobiol Sin 2:279–298 (In Chinese)Google Scholar
- Chen QL, Xie XR (1960) Studies on Sporozoa from the freshwater fishes Ophicephalus maculatus and O. argus of China. Acta Hydrobiol Sin 2:171–196 (In Chinese)Google Scholar
- Fang CL, Chen WJ, Zhou HM, Zhang YP, Fu PF, He G, Wu B, Wang S (2016) The fish resources and their utilization in Lake Poyang. Jiangsu Agri Sci 44:233–243 (In Chinese)Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- He XJ (1988) A preliminary study on Thelohaniasis of Penaeus penlicillatus. J Tropi Oceanol 1:102–106 (In Chinese)Google Scholar
- He XJ, Li ZF (1985) Pleistophora pseudorasborae, a new species of Microsporidia (Sporozoa: Myxosporidia). Acta Zootaxon Sin 10:234–238 (In Chinese)Google Scholar
- Kent ML, Shaw RW, Sanders JL (2014) Microsporidia in fish. 2014. In: Weiss LM, Becnel JJ (eds) Microsporidia: pathogens of opportunity, 1st edn. Wiley Blackwell, Oxford, pp 493–520Google Scholar
- Page RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358Google Scholar
- Simakova A, Tokarev YS, Issi IV (2018) A new microsporidium Fibrilla daphinae g. n. sp. n. infecting Daphnia magna (Crustacea: Cladocera) in Siberia and its taxonomic placing within a new family Fibrillasporidae and new superfamily Tubulinosematidea (Opisthosporidia: Microsporidia). Parasitol Res 117:759–766CrossRefGoogle Scholar
- Tourtip S, Wongtripop S, Stentiford GD, Bateman KS, Sriurairarana S, Chavadej J, Sritunyalucksana K, Withyachumnarnkul B (2009) Enterocytozoon hepatopenaei sp. nov. (Microsporidia: Enterocytozoonidae) a parasite of the black tiger shrimp Penaeus monodon (Decapoda: Penaeidae) fine structure and phylogenetic relationships. J Invertebr Pathol 102:21–29CrossRefGoogle Scholar
- Wu HB, Wu YS, Wu ZH (2005) Occurence of a new Microspordium in the abdominal cavity of Epinephelus akaara. Acta Hydrobiol Sin 29:150–154 (In Chinese)Google Scholar
- Yan YY, Liu XH, Xu LW, Zhang JY (2018) The taxonomic position of causative agent of enteric microsporidiosis of hatchery-bred juvenile grouper, Epinephelus spp., cultured in the area off coast of South China Sea. Acta Hydrobiol Sin 42:947–953 (In Chinese)Google Scholar