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Neurocysticercosis serodiagnosis: mimotope-based synthetic peptide as potential biomarker

  • Vanessa da Silva RibeiroEmail author
  • Henrique Tomaz Gonzaga
  • Daniela da Silva Nunes
  • Luiz Ricardo Goulart
  • Julia Maria Costa-Cruz
Immunology and Host-Parasite Interactions - Short Communication
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Abstract

Herein, we evaluate a mimotope-based synthetic peptidenamed NC41 to diagnose neurocysticercosis (NC), a neglected parasitic disease and a major cause of epilepsy worldwide. NC41 synthetic peptide was evaluated to diagnose NC, and total saline extract from Taenia solium metacestodes (SE) was used as control. Serum samples from patients with NC (n = 40), other parasitic diseases (n = 43), and healthy individuals (n = 40) were tested. Diagnostic parameters such as sensitivity (Se), specificity (Sp), likelihood ratio (LR), and area under curve (AUC) were calculated using receiver operating characteristic (ROC) curves. The sequence from T. solium phosphoenolpyruvate carboxykinase (PEPCK) was used for epitope prediction, resulting in one high-scoring patch centered at residue L247. NC41 synthetic peptide reached high diagnostic performance (Se 97.5% and Sp 97.5%, LR+ 39 and AUC 0.997). Data from diagnostic parameters and in silico analyses proved the usefulness of NC41 synthetic peptide as a diagnostic marker for human NC.

Keywords

ELISA Epitope Prediction NC4Serodiagnosis Synthetic peptide 

Notes

Funding information

This study was supported by the Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—no. 3200601004M-8), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG—no. CBB-PPM-00396-13), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—no. 302426/2012-4).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Research Ethics Committee of the Universidade Federal de Uberlândia (UFU), Minas Gerais state, Brazil (approved under protocol number 041/09), and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Supplementary material

436_2019_6288_Fig2_ESM.png (2 mb)
Supplementary material 1

A: Average score profile of NC41 synthetic peptide in a patch centered on the surface-exposed residues of Taenia solium phosphoenolpyruvate carboxykinase (PEPCK) protein surface. B–D: Results of conformational predicted epitope residues (color) on PECK, in detail (B) and in different views (C–D). Amino acids predicted by the EpiSearch method present in the highest scoring patch centered on L247 and also presented in the peptide sequence—match residues: M219, T222, T242, N243, M244, L247, T248, P249, L251, W254, M257, T294, P296, N297, W468, F482, and N484; predicted length 17aa. (TIF 4469 kb) (PNG 2051 kb)

436_2019_6288_MOESM1_ESM.TIF
High resolution image (TIF 4469 kb)

References

  1. da Silva Ribeiro V, Manhani MN, Cardoso R, Vieira CU, Goulart LR, Costa-Cruz JM (2010) Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis. Immunol Lett 129:94–99.  https://doi.org/10.1016/j.imlet.2010.01.008 CrossRefPubMedGoogle Scholar
  2. Del Brutto OH (2012) Diagnostic criteria for neurocysticercosis, revisited. Pathog Glob Health 106:299–304.  https://doi.org/10.1179/2047773212Y.0000000025 CrossRefPubMedPubMedCentralGoogle Scholar
  3. Elisei RMT, Matos CS, Carvalho AMRS, Chaves AT, Medeiros FAC, Barbosa R, Marcelino AP, dos Santos Emidio K, Coelho EAF, Duarte MC, de Oliveira Mendes TA, da Costa Rocha MO, Menezes-Souza D (2018) Immunogenomic screening approach to identify new antigens for the serological diagnosis of chronic Chagas’ disease. Appl Microbiol Biotechnol 102:6069–6080.  https://doi.org/10.1007/s00253-018-8992-7 CrossRefGoogle Scholar
  4. FAO/WHO [Food and Agriculture Organization of the United Nations/World Health Organization] (2014) Multicriterial-based ranking for risk management of food-borne parasites. Microbiologycal Risk Assessment Series No 23. Rome. 302ppGoogle Scholar
  5. Feliciano ND, Ribeiro VS, Gonzaga HT, Santos FA, Fujimura PT, Goulart LR, Costa-Cruz JM (2016) Short epitope-based synthetic peptides for serodiagnosis of human strongyloidiasis. Immunol Lett 172:89–93.  https://doi.org/10.1016/j.imlet.2016.03.002 CrossRefPubMedGoogle Scholar
  6. Garcia HH, Nash TE, Del Brutto OH (2014) Clinical symptoms, diagnosis, and treatment of neurocysticercosis. Lancet Neurol 13:1202–1215.  https://doi.org/10.1016/S1474-4422(14)70094-8 CrossRefPubMedPubMedCentralGoogle Scholar
  7. Gómara MJ, Haro I (2007) Synthetic peptides for the immunodiagnosis of human diseases. Curr Med Chem 14:531–546.  https://doi.org/10.2174/092986707780059698 CrossRefPubMedGoogle Scholar
  8. Hernández-González A, Noh J, Perteguer MJ, Gárate T, Handali S (2017) Comparison of T24H-his, GST-T24H and GST-Ts8B2 recombinant antigens in western blot, ELISA and multiplex bead-based assay for diagnosis of neurocysticercosis. Parasit Vectors 10:237–248.  https://doi.org/10.1186/s13071-017-2160-2 CrossRefPubMedPubMedCentralGoogle Scholar
  9. Lage DP, Martins VT, Duarte MC, Costa LE, Garde E, Dimer LM, Kursancew AC, Chávez-Fumagalli MA, de Magalhães-Soares DF, Menezes-Souza D, Roatt BM, Machado-de-Ávila RA, Soto M, Tavares CA, Coelho EA (2016) A new Leishmania-specific hypothetical protein and its non-described specific B cell conformational epitope applied in the serodiagnosis of canine visceral leishmaniasis. Parasitol Res 115:1649–1658.  https://doi.org/10.1007/s00436-016-4904-x CrossRefPubMedGoogle Scholar
  10. Leung NY, Wai CY, Ho MH, Liu R, Lam KS, Wang JJ, Shu SA, Chu KH, Leung PS (2017) Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one-compound peptide libraries. Cell Mol Immunol 14:308–318.  https://doi.org/10.1038/cmi.2015.83 CrossRefPubMedGoogle Scholar
  11. List C, Qi W, Maag E, Gottstein B, Müller N, Felger I (2010) Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray. PLoS Negl Trop Dis 4:e771.  https://doi.org/10.1371/journal.pntd.0000771 CrossRefPubMedPubMedCentralGoogle Scholar
  12. Meshgi B, Jalousian F, Fathi S, Jahani Z (2018) Design and synthesis of a new peptide derived from Fasciola gigantica cathepsin L1 with potential application in serodiagnosis of fascioliasis. Exp Parasitol 189:76–86.  https://doi.org/10.1016/j.exppara.2018.04.013 CrossRefPubMedGoogle Scholar
  13. Nash TE, Mahanty S, Garcia HH (2013) Neurocysticercosis-more than a neglected disease. PLoS Negl Trop Dis 7:e1964.  https://doi.org/10.1371/journal.pntd.0001964 CrossRefPubMedPubMedCentralGoogle Scholar
  14. Noh J, Rodriguez S, Lee YM, Handali S, Gonzalez AE, Gilman RH, Tsang VC, Garcia HH, Wilkins PP (2014) Recombinant protein and synthetic peptide-based immunoblot test for diagnosis of neurocysticercosis. J Clin Microbiol 52:1429–1434.  https://doi.org/10.1128/JCM.03260-13 CrossRefPubMedPubMedCentralGoogle Scholar
  15. Noya O, Patarroyo ME, Guzman F, Alarcón de Noya B (2003) Immunodiagnosis of parasitic diseases with synthetic peptides. Curr Protein Pept Sci 4:299–308.  https://doi.org/10.2174/1389203033487153 CrossRefPubMedGoogle Scholar
  16. Nunes DS, Gonzaga HT, Ribeiro VS, Cunha-Júnior JP, Costa-Cruz JM (2017) Usefulness of gel filtration fraction as potential biomarker for neurocysticercosis in serum: towards a new diagnostic tool. Parasitology 144:426–435.  https://doi.org/10.1017/S0031182016001839 CrossRefPubMedGoogle Scholar
  17. Ribeiro VS, Araújo TG, Gonzaga HT, Nascimento R, Goulart LR, Costa-Cruz JM (2013) Development of specific scFv antibodies to detect neurocysticercosis antigens and potential applications in immunodiagnosis. Immunol Lett 156:59–67.  https://doi.org/10.1016/j.imlet.2013.09.005 CrossRefGoogle Scholar
  18. Sotelo J, Guerrero V, Rubio F (1985) Neurocysticercosis: a new classification based on active and inactive forms. A study of 753 cases. Arch Intern Med 145:442–445.  https://doi.org/10.1001/archinte.1985.00360030074016 CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Vanessa da Silva Ribeiro
    • 1
    Email author
  • Henrique Tomaz Gonzaga
    • 1
  • Daniela da Silva Nunes
    • 1
  • Luiz Ricardo Goulart
    • 2
  • Julia Maria Costa-Cruz
    • 1
  1. 1.Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências BiomédicasUniversidade Federal de UberlândiaUberlândiaBrazil
  2. 2.Laboratório de Nanobiotecnologia, Instituto de Genética e BioquímicaUniversidade Federal de UberlândiaUberlândiaBrazil

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