First report of molecular characterization and phylogenetic analysis of Sarcocystis tenella from India
- 10 Downloads
Sarcocystis tenella is a common tissue coccidian parasite of sheep. It is reported worldwide with high prevalence rate ranging from 9 to 100%. However, there are very limited reports of this parasite from the Indian context and those reports are totally based on the morphology alone. When it comes to molecular characterization, such studies are absent from India. The present communication reports the first characterization study of S. tenella from India. 18S rRNA ribosomal gene and mitochondrial cytochrome c oxidase subunit I (cox1) genes were used for molecular characterization and phylogenetic analysis alongside standard histopathology of sarcocysts. Five Indian isolates were characterized for each gene, and respective sequences were submitted in the NCBI. Two haplotypes were noticed, both for the 18S rRNA and cox1 gene showing 99.8–100.0% and 99.7–100.0% nucleotide homologies within themselves, respectively. When compared with other sequences of S. tenella across the globe, the present isolates showed 93.3–99.9% nucleotide homology based on 18S rRNA gene and 95.2–99.8% nucleotide homology based on cox1 gene, respectively. In both the 18S and cox1 phylogenetic trees, respective sequences of S. tenella were placed with monophyletic cluster which was sister to a cluster comprising of sequences of S. gracilis and S. alces.
KeywordsSarcocystis tenella 18S rRNA cox1 Molecular characterization Phylogenetic analysis
The authors are highly thankful to the Director of Research, Dean, and Vice Chancellor of DUVASU, for the facilities provided. The authors also want to acknowledge the various funding agencies like Indian Council of Agricultural Research (ICAR) and Rashtriya Krishi Vikas Yojana (RKVY) for sanctioning various projects to the University for the procurement of instruments to carry out such work. The authors also acknowledge the Incharge of Central Instrumentation Facility (CIF) for using the instruments.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Chhabra MB, Samantaray S (2013) Sarcocystis and sarcocystosis in India: status and emerging perspectives. J Parasit Dis 37(1):1–10Google Scholar
- Dubey JP, Speer CA, Fayer R (1989) Sarcocystosis of animals and man, 1st edn. CRC Press, Boca Raton, p 215Google Scholar
- Dubey JP, Calero-Bernal R, Rosenthal BM, Speer CA, Fayer R (2016) Sarcocystosis of animals and humans, 2nd edn. CRC Press, Boca Raton, pp 195–214 217–234, 243–248, 273–275Google Scholar
- Gjerde B (2013) Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. Int J Parasitol 43:579–591. https://doi.org/10.1016/j.ijpara.2013.02.004
- Gopal K, Pazhanival N, Kumar V, Thangathurai (2016) Some observations on Sarcocystis infection in sheep. Indian Vet J 90(10):81–82Google Scholar
- Hu JJ, Huang S, Wen T, Esch GW, Liang Y, Li HL (2017) Sarcocystis spp. in domestic sheep in Kunming City, China: prevalence, morphology, and molecular characteristics. Parasite 24:30Google Scholar
- Kolenda R, Ugorski M, Bednarski M (2014) Molecular characterization of Sarcocystis species from Polish roe deer based on ssurRNA and cox1 sequence analysis. Parasitol Res 113:3029–3039. https://doi.org/10.1007/s00436-014-3966-x
- Sudan V, Shanker D (2018) Redescription of Sarcocystis species affecting buffaloes in the wake of advancement in molecular biology in parasitology: camera lucida to OMICS. Himanshu Publishers, Udaipur, pp 133–141Google Scholar
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739. https://doi.org/10.1093/molbev/msr121
- Wang G, Wei T, Wang X, Li W, Zhang P, Dong M, Xiao H (1988) The morphology and life cycle of Sarcocystismicrops n. sp. in sheep of Qinghai in China. China Vet Technol 6:9–11Google Scholar