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First report of molecular characterization and phylogenetic analysis of Sarcocystis tenella from India

  • Vikrant SudanEmail author
  • Rahul Kumar
  • Daya Shanker
  • Sanjhi Paliwal
Genetics, Evolution, and Phylogeny - Original Paper
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Abstract

Sarcocystis tenella is a common tissue coccidian parasite of sheep. It is reported worldwide with high prevalence rate ranging from 9 to 100%. However, there are very limited reports of this parasite from the Indian context and those reports are totally based on the morphology alone. When it comes to molecular characterization, such studies are absent from India. The present communication reports the first characterization study of S. tenella from India. 18S rRNA ribosomal gene and mitochondrial cytochrome c oxidase subunit I (cox1) genes were used for molecular characterization and phylogenetic analysis alongside standard histopathology of sarcocysts. Five Indian isolates were characterized for each gene, and respective sequences were submitted in the NCBI. Two haplotypes were noticed, both for the 18S rRNA and cox1 gene showing 99.8–100.0% and 99.7–100.0% nucleotide homologies within themselves, respectively. When compared with other sequences of S. tenella across the globe, the present isolates showed 93.3–99.9% nucleotide homology based on 18S rRNA gene and 95.2–99.8% nucleotide homology based on cox1 gene, respectively. In both the 18S and cox1 phylogenetic trees, respective sequences of S. tenella were placed with monophyletic cluster which was sister to a cluster comprising of sequences of S. gracilis and S. alces.

Keywords

Sarcocystis tenella 18S rRNA cox1 Molecular characterization Phylogenetic analysis 

Notes

Acknowledgements

The authors are highly thankful to the Director of Research, Dean, and Vice Chancellor of DUVASU, for the facilities provided. The authors also want to acknowledge the various funding agencies like Indian Council of Agricultural Research (ICAR) and Rashtriya Krishi Vikas Yojana (RKVY) for sanctioning various projects to the University for the procurement of instruments to carry out such work. The authors also acknowledge the Incharge of Central Instrumentation Facility (CIF) for using the instruments.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

436_2019_6282_MOESM1_ESM.doc (106 kb)
ESM 1 (DOC 105 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Vikrant Sudan
    • 1
    Email author
  • Rahul Kumar
    • 1
  • Daya Shanker
    • 1
  • Sanjhi Paliwal
    • 1
  1. 1.College of Veterinary Sciences &Animal HusbandryU. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU)MathuraIndia

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