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Parasitology Research

, Volume 118, Issue 5, pp 1393–1402 | Cite as

On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters

  • Gokmen Zafer PekmezciEmail author
  • Banu Yardimci
Fish Parasitology - Original Paper

Abstract

Anisakis and Contracaecum species are fish borne zoonotic nematodes. In our previous studies, other larval anisakid and raphidascarid nematodes, Anisakis and Hysterothylacium species, were genetically identified in marine fish from Turkish waters. However, there is no information on molecular identification of larval Contracaecum species in marine fish from Turkey. Therefore, the aim of this study was only to investigate the presence and molecular identification of Contracaecum species in commonly commercialized marine fish from Turkish waters. A total of 475 marine fish, which belong to 21 different species, were sampled from the Aegean (FAO 37.3.1), Mediterranean (FAO 37.3.2), and Black Sea (FAO 37.4.2). The prevalence of Contracaecum L3 larvae in the Aegean Sea was identified as 10% in Mugil cephalus. All Contracaecum L3 larvae were molecularly characterized with RFLP targeting the ITS region and rrnS gene. Moreover, all larvae were analyzed by sequencing of ITS region, rrnS and cox2 gene. All Contracaecum larvae were identified as C. overstreeti based on the cox2 sequence analysis. This is the first report of C. overstreeti larvae in M. cephalus as paratenic and intermediate hosts. Furthermore, the analysis reveals novel information on ITS region. Additionally, the rrnS gene of C. overstreeti was also achieved and deposited in Genbank for the first time. The PCR-RFLP patterns of the ITS region and rrnS gene from C. overstreeti were presented in the present study. Consequently, the presence of C. overstreeti larvae in M. cephalus from the Aegean Sea may also potentially capable of inducing allergic sensitization in humans. 

Keywords

Contracaecum overstreeti ITS region cox2 rrnS Mugil cephalus  Turkish waters 

Notes

Acknowledgments

We would like to thank Associate Professor Didem Pekmezci (University of Ondokuz Mayis, Turkey) and Oyku Naz Attila (University of Strathclyde, Glasgow, UK) for their help in English proofreading.

Funding information

This study was supported by The Scientific Research Council of the University Ondokuz Mayis, Samsun, Turkey (project number PYO.VET.1902.15.002).

Supplementary material

436_2019_6278_MOESM1_ESM.docx (26 kb)
ESM 1 (DOCX 26.0 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Aquatic Animal Diseases, Faculty of Veterinary MedicineOndokuz Mayis UniversitySamsunTurkey

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