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Parasitology Research

, Volume 118, Issue 5, pp 1493–1500 | Cite as

Oral infection of mice and host cell invasion by Trypanosoma cruzi strains from Mexico

  • Cecilia G. Barbosa
  • César Gómez-HernándezEmail author
  • Karine Rezende-Oliveira
  • Marcos Vinicius Da Silva
  • João Paulo Ferreira Rodrigues
  • Monique G. S. Tiburcio
  • Thatiane Bragini Ferreira
  • Virmondes Rodrigues
  • Nobuko Yoshida
  • Luis E. Ramirez
Immunology and Host-Parasite Interactions - Original Paper

Abstract

Oral infection by Trypanosoma cruzi has been responsible for frequent outbreaks of acute Chagas disease in the north of South America and in the Amazon region, where T. cruzi genetic group TcI predominates. TcI strains from different geographical regions have been used in oral infection in mice, but there is no information about strains from Mexico where TcI is prevalent. Here, we analyzed four Mexican strains as concerns the course of oral infection, the ability to invade host cells in vitro, and the profile of metacyclic trypomastigote surface molecules gp82 and gp90 that are implicated in parasite internalization. Oral infection of mice with metacyclic forms of all strains resulted in reduced blood and tissue parasitism, and mild to moderate inflammatory process in the heart/skeletal muscle. They expressed pepsin-resistant gp82 and gp90 molecules at high levels and invaded host cells poorly in full nutrient medium and efficiently under nutrient-deprived condition. The properties exhibited by Mexican strains were similar to those displayed by TcI strains from other geographical regions, reinforcing the notion that these features are common to the genetic group TcI as a whole.

Keywords

Trypanosoma cruzi Genetic group TcI Oral infection Mexican strains Metacyclic trypomastigote Surface molecules gp82 and gp90 

Notes

Acknowledgments

The authors thank Dr. Fernando Yukio Maeda for the help in some assays.

Financial support

This work was supported by the National Incentive Program for Research in Parasitology Basic/2010, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) process 307284/ 2014-0 (research productivity fellowship).

Compliance with ethical standards

The study was approved by the Ethics Committee on Animal Experimentation of Universidade Federal do Triângulo Mineiro (UFTM), protocol number 308. We have followed the guidelines that recommend the use of anesthetic agents, such as ketamine/xylazine, to euthanize animals.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Cecilia G. Barbosa
    • 1
  • César Gómez-Hernández
    • 1
    Email author
  • Karine Rezende-Oliveira
    • 2
  • Marcos Vinicius Da Silva
    • 1
  • João Paulo Ferreira Rodrigues
    • 3
  • Monique G. S. Tiburcio
    • 1
  • Thatiane Bragini Ferreira
    • 1
  • Virmondes Rodrigues
    • 1
  • Nobuko Yoshida
    • 3
  • Luis E. Ramirez
    • 1
  1. 1.Universidade Federal do Triângulo MineiroUberabaBrazil
  2. 2.Universidade Federal de UberlândiaItuiutabaBrazil
  3. 3.Universidade Federal de São PauloSão PauloBrazil

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