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Parasitology Research

, Volume 118, Issue 2, pp 617–630 | Cite as

Molecular characterization of South Indian field isolates of bovine Babesia spp. and Anaplasma spp.

  • Rangapura Kariyappa Pradeep
  • Murikoli Nimisha
  • Meethalae Koombayil Sruthi
  • Pakideery Vidya
  • Birur Mallappa Amrutha
  • Prashant Somalingappa Kurbet
  • Karapparambu Gopalan Ajith Kumar
  • Anju Varghese
  • Chundayil Kalarikkal Deepa
  • Chemmangattuvalappil Narendranath Dinesh
  • Leena Chandrasekhar
  • Sanis Juliet
  • Puthenparambil Ramakrishnan Pradeepkumar
  • Chintu Ravishankar
  • Srikant Ghosh
  • Reghu RavindranEmail author
Protozoology - Original Paper

Abstract

Ticks and tick-borne diseases (TTBDs) are considered major causes of economic loss in the livestock sector which incur an annual control cost estimated at US$ 498.7 million in India. Among these diseases, babesiosis, theileriosis and anaplasmosis are listed among the top ten livestock diseases in India and cause significant mortality and morbidity among cattle. However, molecular characterization of bovine Babesia and Anaplasma species are scant; thus, the aim of this study is to perform molecular characterization of field isolates of Babesia spp. and Anaplasma spp. infecting bovines in Kerala, South India. Blood smears and whole blood samples were collected from a total of 199 apparently healthy adult female cattle in Kerala. Based on microscopy, Babesia spp., Theileria orientalis and Anaplasma spp. organisms were detected in 9 (4.5%), 40 (20%) and 6 (3%) samples, respectively. Genus-specific polymerase chain reactions for amplification of 18S rRNA of Babesia spp. and 16S rRNA of Anaplasma spp. revealed positive results with 18 (9%) and 14 (7%) samples. The phylogenetic analysis of 18S rRNA gene sequences of Babesia spp. confirmed the existence of two different populations of Babesia spp. circulating in the blood of infected cattle viz., Babesia bigemina and a Babesia sp. genetically related to Babesia ovata. Further phylogenetic analysis using rap-1a sequences of isolates of B. bigemina revealed higher levels of genetic heterogeneity. However, the field isolates of B. bigemina displayed only slight heterogeneity when the rap-1c gene was examined. Polymerase chain reaction followed by sequencing and phylogenetic analysis of 16S rRNA gene of Anaplasma spp. revealed the existence of Anaplasma marginale, Anaplasma bovis and Anaplasma platys in bovines in South India. Based on msp4 gene sequences, all the field isolates of A. marginale from Kerala were clustered in a single clade with others isolated from around the world. To our knowledge, this study forms the first report on occurrence of B. ovata-like parasites and A. platys in cattle from India.

Keywords

Babesia bigemina B. ovata like Anaplasma marginale A. platys A. bovis Phylogeny South India 

Notes

Acknowledgements

We thank all the veterinary doctors and local farming community for their cooperation.

Funding information

This work was supported financially by the Indian Council of Agricultural Research (NAIP/C2066, NFBSFARA/BSA-4004/2013-14, NASF/ABA-6015/2016-17), the Kerala State Council for Science, Technology and Environment (022/YIPB/KBC/2013/CSTE, 010-14/ SARD/13/CSTE) and the Department of Animal Husbandry, Kerala [B2.3858/04/Plg(3) dt.2/2/07, B2.8401/08/Plg dt.19/18/2008].

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Rangapura Kariyappa Pradeep
    • 1
  • Murikoli Nimisha
    • 1
  • Meethalae Koombayil Sruthi
    • 1
  • Pakideery Vidya
    • 1
  • Birur Mallappa Amrutha
    • 1
  • Prashant Somalingappa Kurbet
    • 1
  • Karapparambu Gopalan Ajith Kumar
    • 1
  • Anju Varghese
    • 1
  • Chundayil Kalarikkal Deepa
    • 1
  • Chemmangattuvalappil Narendranath Dinesh
    • 2
  • Leena Chandrasekhar
    • 3
  • Sanis Juliet
    • 4
  • Puthenparambil Ramakrishnan Pradeepkumar
    • 5
  • Chintu Ravishankar
    • 6
  • Srikant Ghosh
    • 7
  • Reghu Ravindran
    • 1
    Email author
  1. 1.Department of Veterinary ParasitologyCollege of Veterinary and Animal SciencesWayanadIndia
  2. 2.Department of Animal Breeding and GeneticsCollege of Veterinary and Animal SciencesWayanadIndia
  3. 3.Department of Veterinary AnatomyCollege of Veterinary and Animal SciencesWayanadIndia
  4. 4.Department of Veterinary Pharmacology and ToxicologyCollege of Veterinary and Animal SciencesWayanadIndia
  5. 5.Department of Animal HusbandryThiruvananthapuramIndia
  6. 6.Department of Veterinary MicrobiologyCollege of Veterinary and Animal SciencesWayanadIndia
  7. 7.Division of ParasitologyIndian Veterinary Research InstituteIzatnagarIndia

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