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Parasitology Research

, Volume 118, Issue 2, pp 453–460 | Cite as

Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) interacting with buffalo serum of different infection periods by shotgun LC-MS/MS

  • Si-Yang HuangEmail author
  • Dong-Mei Yue
  • Jun-Ling Hou
  • Xiao-Xuan Zhang
  • Fu-kai Zhang
  • Chun-Ren Wang
  • Xing-Quan ZhuEmail author
Genetics, Evolution, and Phylogeny - Original Paper
  • 106 Downloads

Abstract

Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is an important zoonotic disease in the world. It affects livestock, especially for sheep and cattle, causing major economic loss due to morbidity and mortality. Although the excretory and secretory products (ESPs) of F. hepatica have been relatively well studied, little is known about the interaction between the ESP and host, and the mechanism of the key proteins involved in interaction. In this study, buffaloes were infected by Fasciola gigantica, and infection serum was collected at three different periods (42dpi, 70dpi, and 98dpi). The interaction proteins were pulled down with three different period serum by Co-IP assay, respectively, and then identified by LC-MS/MS analysis. A number of proteins were identified; some of them related to the biological function of the parasite, while most of them the functions were unknown. For the annotated proteins, 13, 5, and 7 proteins were pulled down by the infected serum in 42dpi, 70dpi, and 98dpi, respectively, and 18 proteins could be detected in all three periods. Among them, 13 belong to the cathepsin family, 4 proteins related to glutathione S-transferase, and 3 proteins are calcium-binding protein; other proteins related to catalytic activity and cellular process. This study could provide new insights into the central role played by ESPs in the protection of F. gigantica from the host immune response. At the same time, our research provided material for further studies about the interaction between F. gigantica and host.

Keywords

Fasciola gigantica Excretory and secretory products (ESPs) Infection Co-IP Mass spectrometry 

Notes

Acknowledgments

Project support was provided by the “National Key Basic Research Program (973 Program) of China” (Grant No. 2015CB150300), the National Key Research and Development Program of China (Grant No. 2017YFD0501200), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (Veterinary Medicine); The authors thank Shanghai Applied Protein Technology Co. Ltd., for technical assistance in LC-MS/MS analysis.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Supplementary material

436_2018_6169_MOESM1_ESM.xlsx (17 kb)
ESM 1 (XLSX 17 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Si-Yang Huang
    • 1
    Email author
  • Dong-Mei Yue
    • 2
    • 3
  • Jun-Ling Hou
    • 2
  • Xiao-Xuan Zhang
    • 3
  • Fu-kai Zhang
    • 2
  • Chun-Ren Wang
    • 3
  • Xing-Quan Zhu
    • 1
    • 2
    Email author
  1. 1.Institute of Comparative MedicineYangzhou University, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, and Jiangsu Key Laboratory of ZoonosisYangzhouPeople’s Republic of China
  2. 2.State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouPeople’s Republic of China
  3. 3.College of Animal Science and Veterinary MedicineHeilongjiang Bayi Agricultural UniversityDaqingPeople’s Republic of China

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