Parasitology Research

, Volume 118, Issue 1, pp 245–253 | Cite as

Molecular detection of benzimidazole resistance levels associated with F167Y and F200Y polymorphisms in Haemonchus contortus of goats from Mozambique

  • Alsácia Atanásio-NhacumbeEmail author
  • Sabrina Mota Lambert
  • Bárbara Maria Paraná da Silva Souza
  • Maria Consuêlo Carybé Ayres
Helminthology - Original Paper


Benzimidazole (BZ) resistance of Haemonchus contortus has been associated with single nucleotide polymorphisms (SNPs) in codons 200 (F200Y) and 167 (F167Y) and, to a lesser extent, in codon E198A, of the β-tubulin isotype 1 gene. The present study was undertaken to survey the status of BZ resistance in naturally infected goats in smallholder farms in southern Mozambique by real-time PCR (qPCR) using TaqMan® assays. H. contortus-infective larvae (L3; n = 432) from 12 populations were individually genotyped for F200Y and F167Y SNPs to detect BZ resistance. For the F200Y SNP, the results revealed an overall mean percentages of 18.8% homozygous resistant (RR), 47.8% homozygous susceptible (SS) and 33.4% heterozygous (RS) H. contortus. For the F167Y SNP, the overall mean percentages were 1.6% RR, 94.9% SS and 3.5% RS. The percentage of resistant alleles (%R) for the F200Y and F167Y SNPs was 35.7 and 3.4%, respectively. Genotype combinations of the two mutations indicate resistant percentages ranging from 0.0 to 52.9%. From the four herds with high RR individuals, three farms dewormed the animals monthly, while the fourth farm dewormed the animals every 3 months. In farms where animals were dewormed every 6 months, low percentages of RR individuals were found, whereas no RR individuals were discovered in herds where animals were dewormed annually. These results suggest that the F200Y SNP is more significant in BZ resistance development of the surveyed population compared with the F167Y SNP.


Anthelmintic resistance β-Tubulin Gastrointestinal nematodes Real-time PCR Small ruminants 



This study was supported by the Ministry of Science and Technology, Higher Education, and Technical Vocational Training (MCTESTP) through the High Education, Science and Technology (HEST) Project—World Bank. We thank the technical assistance provided by Dr. Aida Cala, Mr. Carlos Sitoe and Mrs. Ana Paula from the Animal Sciences Directorate (DCA) of the Agricultural Research Institute of Mozambique (IIAM) and by Mr. Gedeão Macanze from the Provincial Veterinary Laboratory in Gaza. We express our sincere appreciation to the IIAM General Director, Dr. Olga Fafetine, and the DCA Director, Dr. Zacarias Massango, for logistical support during the field work. The technical and logistical support provided by the staff from the Molecular and Cellular Biology Laboratory (LBCM) of the Federal University of Bahia (UFBA), Brazil, is especially acknowledged.

Compliance with ethical standards

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. Approval from the Ethical Commission on the Use of Animals at the School of Veterinary Medicine—Federal University of Bahia (UFBA), Brazil, has been registered under EMZV-UFBA No. 09/2017.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Veterinary MedicineFederal University of Bahia (UFBA)SalvadorBrazil
  2. 2.National Centre for Biotechnology and Biosciences (CNBB), Ministry of Science & TechnologyHigh Education and Vocational Training (MCTESTP)MaputoMozambique

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