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Parasitology Research

, Volume 117, Issue 11, pp 3481–3486 | Cite as

Dispersion and infectivity of Toxocara canis eggs after passage through chicken intestine

  • Yslla Fernanda Fitz Balo Merigueti
  • Ricardo da Silva Raposo
  • Bianca Pelegi Zampieri
  • Letícia Maria de Lima Cerazo
  • Ludimilla Pereira
  • Vamilton Alvares Santarém
Original Paper

Abstract

Toxocariasis is an important, but neglected, worldwide zoonosis. It is considered a primarily soil-transmitted disease, but food-borne transmission has been associated with the consumption either of raw or undercooked meat of paratenic hosts, including birds. Despite the number of experimental studies carried out to evaluate the behavior of Toxocara spp. larvae in birds, their role in the dispersion of eggs into the environment remains unclear. Thus, this study aimed to evaluate the potential of broiler chickens to release Toxocara canis eggs into the environment, and the infectivity of eggs after passage through the intestine. Forty commercial broiler chickens, aged 60 days, were randomly distributed into three groups. Groups 1 (n = 16) and 2 (n = 16) were orally infected with 5000 embryonated and 5000 unembryonated T. canis eggs, respectively. Group 3 (n = 8) served as a control. Following infection, fecal samples from each chicken were examined using a centrifuge-sedimentation technique. At 24-h, 72-h, and 7-day post-infection (PI), four chickens each from the G1 and G2 groups, and two from the G3 group were killed. After euthanasia, the intestinal content and liver were collected for recovery of T. canis larvae. Results revealed that broiler chickens have the potential to disperse both embryonated and unembryonated T. canis eggs, following 2- to 6-h PI. In addition, the eggs shed into the feces of the G2 birds, after incubation in laboratorial conditions, were infective when they were tested in a bioassay using mice. In conclusion, broiler chickens have the potential of dispersing Toxocara spp. eggs into the environment and the eggs passed through the intestine are infective after being incubated in experimental conditions.

Keywords

Birds Gallus gallus domesticus Environmental contamination Toxocariasis Zoonosis 

Notes

Acknowledgments

We would like to thank the Conselho Nacional de Pesquisa e Desenvolvimento Científico (CNPq) for a scholarship grant to Y.F.F.B. Merigueti.

Compliance with ethical standards

This study was approved by the Ethics Committee for the Use of Animals of the Universidade do Oeste Paulista (CEUA protocol number 1589), and all of procedures for infection and euthanasia were based on Resolution Number 1000 of the Federal Board of Veterinary Medicine (Brazil, 2012).

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals—statement on the welfare of animals

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yslla Fernanda Fitz Balo Merigueti
    • 1
  • Ricardo da Silva Raposo
    • 1
  • Bianca Pelegi Zampieri
    • 2
  • Letícia Maria de Lima Cerazo
    • 2
  • Ludimilla Pereira
    • 2
  • Vamilton Alvares Santarém
    • 1
  1. 1.Post-Graduation Program in Animal Science, Laboratory of Veterinary Parasitology, Veterinary Teaching-HospitalUniversidade do Oeste Paulista (UNOESTE)São PauloBrazil
  2. 2.Laboratory of Veterinary Parasitology, Veterinary Teaching-HospitalUniversidade do Oeste Paulista (UNOESTE)São PauloBrazil

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