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Parasitology Research

, Volume 117, Issue 11, pp 3381–3389 | Cite as

A putative calcium-ATPase of the secretory pathway family may regulate calcium/manganese levels in the Golgi apparatus of Entamoeba histolytica

  • Mario A. Rodríguez
  • Aarón Martínez-Higuera
  • Martha I. Valle-Solis
  • Mario Hernandes-Alejandro
  • Bibiana Chávez-Munguía
  • Ana H. Figueroa-Gutiérrez
  • Andrés Salas-Casas
Original Paper

Abstract

Calcium regulates many cellular processes in protozoa, including growth, differentiation, programmed cell death, exocytosis, endocytosis, phagocytosis, fusion of the endosomes of distinct stages with phagosomes, fusion of phagosomes with lysosomes, and recycling the membrane. In Entamoeba histolytica, the protozoa responsible for human amoebiasis, calcium ions are essential for signaling pathways that lead to growth and development. In addition, calcium is crucial in the modulation of gene expression in this microorganism. However, there is scant information about the proteins responsible for regulating calcium levels in this parasite. In this work, we characterized a protein of E. histolytica that shows a close phylogenetic relationship with Ca2+ pumps that belong to the family of secretory pathway calcium ATPases (SPCA), which for several organisms are located in the Golgi apparatus. The amoeba protein analyzed herein has several amino acid residues that are characteristic of SPCA members. By an immunofluorescent technique using specific antibodies and immunoelectron microscopy, the protein was detected on the membrane of some cytoplasmic vacuoles. Moreover, this putative calcium-ATPase was located in vacuoles stained with NBD C6-ceramide, a Golgi marker. Overall, the current findings support the hypothesis that the presently analyzed protein corresponds to the SPCA of E. histolytica.

Keywords

Entamoeba histolytica Golgi apparatus Calcium-ATPases SPCA 

Notes

Acknowledgments

We thank to Carlos Vázquez-Calzada, for his invaluable technical assistance (Centro de Investigación y de Estudios Avanzados del IPN).

Compliance with ethical standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

436_2018_6030_MOESM1_ESM.docx (60 kb)
ESM 1 (DOCX 59 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mario A. Rodríguez
    • 1
  • Aarón Martínez-Higuera
    • 2
  • Martha I. Valle-Solis
    • 1
  • Mario Hernandes-Alejandro
    • 3
  • Bibiana Chávez-Munguía
    • 1
  • Ana H. Figueroa-Gutiérrez
    • 4
  • Andrés Salas-Casas
    • 4
  1. 1.Departamento de Infectómica y Patogénesis MolecularCentro de Investigación y de Estudios Avanzados del IPNMexico CityMexico
  2. 2.Departamento de FísicaUniversidad de SonoraHermosilloMexico
  3. 3.Departamento de BioingenieríaUnidad Profesional Interdisciplinaria de Biotecnología del Instituto Politécnico Nacional (UPIBI-IPN)Mexico CityMexico
  4. 4.Instituto de Ciencias de la Salud, Área Académica de GerontologíaUniversidad Autónoma del Estado de HidalgoSan Agustín TlaxiacaMexico

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