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Parasitology Research

, Volume 117, Issue 5, pp 1453–1463 | Cite as

First report of Toxoplasma gondii sporulated oocysts and Giardia duodenalis in commercial green-lipped mussels (Perna canaliculus) in New Zealand

  • Alicia CoupeEmail author
  • Laryssa Howe
  • Elizabeth Burrows
  • Abigail Sine
  • Anthony Pita
  • Niluka Velathanthiri
  • Emilie Vallée
  • David Hayman
  • Karen Shapiro
  • Wendi D. Roe
Original Paper

Abstract

Pollution of marine ecosystems with the protozoan parasites Toxoplasma gondii, Cryptosporidium spp. and Giardia duodenalis can be studied using bivalve shellfish as biosentinels. Although evidence suggests that these parasites are present in New Zealand coastal waters, the extent of protozoal pollution has not been investigated. This study used optimised molecular methods to detect the presence of Cryptosporidium spp., G. duodenalis and T. gondii in commercially sourced green-lipped mussel (Perna canaliculus), an endemic species found throughout coastal New Zealand. A nested polymerase chain reaction was validated for detection of T. gondii DNA and applied to 104 commercially sourced mussels. Thirteen mussels were positive for T. gondii DNA with an estimated true prevalence of 16.4% using Bayesian statistics, and the presence of T. gondii in mussels was significantly associated with collection during the summer compared with that in the winter (P = 0.003). Consumption of contaminated shellfish may also pose a health risk for humans and marine wildlife. As only sporulated T. gondii oocysts can be infectious, a reverse transcriptase-polymerase chain reaction was used to confirm presence of a sporozoite-specific marker (SporoSAG), detected in four mussels. G. duodenalis assemblage B, known to be pathogenic in humans, was also discovered in 1% mussels, tested by polymerase chain reaction (n = 90). Cryptosporidium spp. was not detected in the sampled mussel haemolymph. Results suggest that New Zealand may have high levels of coastal contamination with T. gondii, particularly in summer months, and that naturally exposed mussels can ingest and retain sporulated oocysts, further establishing shellfish consumption as a health concern.

Keywords

Biosentinels Toxoplasma gondii Giardia duodenalis SporoSAG Perna canaliculus 

Notes

Acknowledgements

We thank Heather Fritz, Jeroen Saeij and David Arranz Solis for producing and generously providing T. gondii oocysts from the University of California, Davis. We are very grateful to Patricia Conrad, University California, Davis, for supporting collaboration with Massey University and also to Juan-Carlos Garcia Ramirez for providing scientific guidance on Giardia duodenalis in New Zealand.

Funding information

Funding for this work was provided by the New Zealand Department of Conservation, the Massey University Research Foundation, the Lewis Fitch Foundation, the Marian Cunningham Memorial Fund and the New Zealand Ministry of Health. We acknowledge the financial support received from the IVABS Doctoral Scholarship, Massey University and the New Zealand International Doctoral Research Scholarship, Education New Zealand.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Alicia Coupe
    • 1
    Email author
  • Laryssa Howe
    • 1
  • Elizabeth Burrows
    • 1
  • Abigail Sine
    • 1
  • Anthony Pita
    • 2
  • Niluka Velathanthiri
    • 2
  • Emilie Vallée
    • 1
  • David Hayman
    • 2
  • Karen Shapiro
    • 3
    • 4
  • Wendi D. Roe
    • 1
  1. 1.Institute of Veterinary, Animal and Biomedical Sciences, College of SciencesMassey UniversityPalmerston NorthNew Zealand
  2. 2.Molecular Epidemiology and Public Health Laboratory, Hopkirk Research InstituteMassey UniversityPalmerston NorthNew Zealand
  3. 3.One Health Institute, School of Veterinary MedicineUniversity of CaliforniaDavisUSA
  4. 4.Department of Pathology, Microbiology and Immunology, School of Veterinary MedicineUniversity of CaliforniaDavisUSA

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