Parasitology Research

, Volume 117, Issue 4, pp 1285–1289 | Cite as

New records and host associations of the tick Ixodes apronophorus and the first detection of Ehrlichia sp. HF in Romania

  • Martin O. Andersson
  • Gabriel Radbea
  • Dimitrios Frangoulidis
  • Herbert Tomaso
  • Franz Rubel
  • Santiago Nava
  • Lidia Chitimia-DoblerEmail author
Short Communication


Ixodes (Ixodes) apronophorus is a neglected tick species and its geographical distribution, host associations, and role as a disease vector are not well known. We collected I. apronophorus from several locations in Romania. Morphological identification of ticks was confirmed by analysis of 16S rDNA and 12S rDNA gene sequences. We report new host associations of I. apronophorus, which was collected from dogs, foxes, and a hare—all new hosts for this tick species in Romania. Furthermore, we report for the first time occurrence of Ehrlichia sp. HF in I. apronophorus. Ehrlichia sp. HF was identified by sequencing a part of the 16S rDNA gene and was found in 16% (3/19) of the tested ticks. Ehrlichia sp. HF has not been previously reported in Eastern Europe and seems to have a much larger geographic distribution than previously known. Currently, it is unknown whether I. apronophorus is a competent vector for Ehrlichia sp. HF, or if the findings in this study represent infection in the hosts, namely dogs and fox.


Ixodes apronophorus Ehrlichia sp. HF Dogs Romania 



We thank Professor Martin Pfeffer for valuable comments on the manuscript and Byrgit Hofmann for excellent technical assistance. We thank Michael Levin for the help in language.

Funding information

This work was funded by the German Center of Infection Research (DZIF) (LCD) and by the Royal Swedish Academy of Agriculture and Forestry (MOA).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

436_2018_5800_MOESM1_ESM.png (1.5 mb)
ESM 1 Supplementary map Ixodes apronophorus ditribution in Romania: red dots – new finding, yellow dot – Feider (1965); blue dot – Mihalca et al. (2012) and Ehrlichia sp. HF locations in Romania represented by white stars (PNG 1525 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Martin O. Andersson
    • 1
  • Gabriel Radbea
    • 2
  • Dimitrios Frangoulidis
    • 3
    • 4
  • Herbert Tomaso
    • 5
  • Franz Rubel
    • 6
  • Santiago Nava
    • 7
  • Lidia Chitimia-Dobler
    • 3
    • 4
    • 8
    Email author
  1. 1.Center for Ecology and Evolution in Microbial Model Systems (EEMiS)Linnaeus UniversityKalmarSweden
  2. 2.Sal-Vet Private Veterinary ClinicsTimisoaraRomania
  3. 3.Bundeswehr Institute of MicrobiologyMunichGermany
  4. 4.German Center of Infection Research (DZIF) Partner MunichMunichGermany
  5. 5.Friedrich-Loeffler-Institut, Institute of Bacterial Infections and ZoonosesJenaGermany
  6. 6.Institute for Veterinary Public HealthUniversity of Veterinary Medicine ViennaViennaAustria
  7. 7.Estación Experimental Agropecuaria Rafaela, and Consejo Nacional de Investigaciones Científicas y TécnicasInstituto Nacional de Tecnología AgropecuariaBuenos AiresArgentina
  8. 8.Institute for Diagnosis and Animal HealthBucharestRomania

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