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Parasitology Research

, Volume 116, Issue 10, pp 2873–2876 | Cite as

Rare case of acute toxoplasmosis in a domestic rabbit (Oryctolagus cuniculus) in Brazil associated with the type BrIII Brazilian clonal lineage of Toxoplasma gondii

  • Lismara Castro do Nascimento
  • Hilda Fátima Jesus PenaEmail author
  • Ronaldo Viana Leite Filho
  • Fernando Froner Argenta
  • Bruna Farias Alves
  • Solange Oliveira
  • Solange Maria Gennari
  • David Driemeier
Short Communication

Abstract

Toxoplasmosis is a widely distributed disease that infects birds and mammals, including humans. Acute clinical course of toxoplasmosis is considered to be rare among domestic rabbits (Oryctolagus cuniculus). The aim of this study was to present the first report of fatal acute disease caused by Toxoplasma gondii type BrIII genotype, a typical Brazilian clonal lineage, in a domestic rabbit. T. gondii was identified in histological sections of spleen and liver tissue, and these tissues were also immunohistochemically positive for T. gondii. After the histopathological and immunohistochemical confirmation of T. gondii, the genotype of this pathogen was determined via PCR-RFLP with 11 markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3) and via microsatellite (MS) analysis with 15 markers (TUB2, W35, TgMA, B18, B17, M33, IV.1, X1.1, M48, M102, N60, N82, AA, N61, and N83). This study shows that type BrIII genotype, circulating in Brazil in different hosts, can cause acute disease in a naturally infected animal host. The described case also involves the first reported occurrence of the 291 allele for the typing marker TUB2 in a type BrIII strain, emphasizing the genetic diversity of T. gondii in Brazil.

Keywords

Toxoplasma gondii Rabbit breeding PCR-RFLP Microsatellite markers Genetic diversity 

Notes

Acknowledgments

SMG received a fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This study was also supported by a departmental grant from CAPES/PROEX (1841/2016).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Lismara Castro do Nascimento
    • 1
  • Hilda Fátima Jesus Pena
    • 2
    Email author
  • Ronaldo Viana Leite Filho
    • 1
  • Fernando Froner Argenta
    • 1
  • Bruna Farias Alves
    • 2
  • Solange Oliveira
    • 2
  • Solange Maria Gennari
    • 2
  • David Driemeier
    • 1
  1. 1.Department of Pathology and Veterinary Clinic, Faculty of Veterinary MedicineFederal University of Rio Grande do Sul (FaVet/UFRGS)Porto AlegreBrazil
  2. 2.Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal ScienceUniversity of São Paulo (FMVZ/USP)São PauloBrazil

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