Abstract
The effect of anti-CD25 monoclonal antibody (anti-CD25 mAb) on the protection efficacy of Schistosoma japonicum 26 kDa GST (glutathione-S-transferase) vaccine was evaluated. Mice were immunized with GST before infection with S. japonicum cercariae and then injected with anti-CD25 mAb. The worm reduction rate was promoted from 24.18% in mice with GST immunization to 47.09% in mice with GST plus anti-CD25 mAb. Compared with the control group, the percentages of splenic CD4+CD25+Foxp3+ regulatory T cells (Tregs) were significantly lower after administration of anti-CD25 mAb; meanwhile, elevated levels of IFN-γ and IL-2 were secreted by splenocytes. These results indicate that the poor protective efficacy of the GST vaccine against S. japonicum results from the presence of CD4+CD25+Foxp3+ Tregs, while anti-CD25 mAb can partially block CD4+CD25+Foxp3+ Tregs and thus enhance the protective efficacy of the GST vaccine.
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We are grateful to Simone Haeberlein for the advice on article writing.
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The experiment was approved by the Committee on Animal Research of Wuchang Hospital (No. 2014-0013).
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The authors declare that they have no conflict interest.
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This work was funded by the Scientific Research Subject of the Health and Family Planning Commission of Wuhan Municipality (No. WX17A08).
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Tang, Cl., Yang, J., Cheng, Ly. et al. Anti-CD25 monoclonal antibody enhances the protective efficacy of Schistosoma japonicum GST vaccine via inhibition of CD4+CD25+Foxp3+ regulatory T cells. Parasitol Res 116, 2727–2732 (2017). https://doi.org/10.1007/s00436-017-5581-0
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DOI: https://doi.org/10.1007/s00436-017-5581-0