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Parasitology Research

, Volume 116, Issue 4, pp 1273–1284 | Cite as

Update on Acanthamoeba jacobsi genotype T15, including full-length 18S rDNA molecular phylogeny

  • Daniele CorsaroEmail author
  • Martina Köhsler
  • Margherita Montalbano Di Filippo
  • Danielle Venditti
  • Rosa Monno
  • David Di Cave
  • Federica Berrilli
  • Julia Walochnik
Original Paper

Abstract

Free-living amoebae of the genus Acanthamoeba are worldwide present in natural and artificial environments, and are also clinically important, as causative agents of diseases in humans and other animals. Acanthamoeba comprises several species, historically assigned to one of the three groups based on their cyst morphology, but presently recognized as at least 20 genotypes (T1-T20) on the basis of their nuclear 18S ribosomal RNA (rRNA) gene (18S rDNA) sequences. While strain identification may usually be achieved targeting short (<500 bp) 18S ribosomal DNA (rDNA) fragments, the use of full-length gene sequences (>2200 bp) is necessary for correct genotype description and reliable molecular phylogenetic inference. The genotype T15, corresponding to Acanthamoeba jacobsi, is the only genotype described on the basis of partial sequences (~1500 bp). While this feature does not prevent the correct identification of the strains, having only partial sequences renders the genotype T15 not completely defined and may furthermore affect its position in the Acanthamoeba molecular tree. Here, we complete this gap, by obtaining full-length 18S rDNA sequences from eight A. jacobsi strains, genotype T15. Morphologies and physiological features of isolated strains are reported. Molecular phylogeny based on full 18S rDNA confirms some previous suggestions for a genetic link between T15 and T13, T16, and T19, with T19 as sister-group to T15.

Keywords

Acanthamoeba Acanthamoeba jacobsi Acanthamoeba T15 Group III acanthamoebae Group I intron 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.CHLAREAS Chlamydia Research AssociationVandoeuvre-lès-NancyFrance
  2. 2.Molecular Parasitology, Institute of Specific Prophylaxis and Tropical MedicineMedical University of ViennaViennaAustria
  3. 3.Department of Clinical Sciences and Translational MedicineUniversity of Rome “Tor Vergata”RomeItaly
  4. 4.TREDI Research Department, Faculty of MedecineVandoeuvre-lès-NancyFrance
  5. 5.Department of Basic Medical Science, Neuroscience and Sense OrgansUniversity of Bari “Aldo Moro”BariItaly

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