Preliminary genetic evidence of two different populations of Opisthorchis viverrini in Lao PDR
Opisthorchis viverrini is a major public health concern in Southeast Asia. Various reports have suggested that this parasite may represent a species complex, with genetic structure in the region perhaps being dictated by geographical factors and different species of intermediate hosts. We used four microsatellite loci to analyze O. viverrini adult worms originating from six species of cyprinid fish in Thailand and Lao PDR. Two distinct O. viverrini populations were observed. In Ban Phai, Thailand, only one subgroup occurred, hosted by two different fish species. Both subgroups occurred in fish from That Luang, Lao PDR, but were represented to very different degrees among the fish hosts there. Our data suggest that, although geographical separation is more important than fish host specificity in influencing genetic structure, it is possible that two species of Opisthorchis, with little interbreeding, are present near Vientiane in Lao PDR.
KeywordsMicrosatellite DNA Opisthorchis viverrini Population genetics Host factors Cyprinid fish
|Funder Name||Grant Number||Funding Note|
|Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through health cluster (SHeP-GMS)|
|the Thailand Research Fund through the Basic Research Grant and the Royal Golden Jubilee Ph.D. Program|
|the National Institutes of Health|