Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014–2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 °C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.
KeywordsTrichinella patagoniensis Persistence in decomposing muscle tissue Infectivity Guinea pigs Cavia porcellus
This study was supported by grants from MINCyT PICT 2013 – 0965 and UBACyT 20020130100336BA. The research of M.K. and J.I. was financially supported by the The Ministry of Education, Youth and Sports of the Czech Republic (7AMB15AR020 and LH12096), Masaryk University, Brno (MUNI/A/1325/2015) and the Charles University in Prague (PRVOUK P41, UNCE 204017 and SVV 260202/2015).
Compliance with ethical standards
The present research was performed in compliance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and was approved by the Committee for the Use and Care of Laboratory Animals (CICUAL) of the Facultad de Ciencias Veterinarias, University of Buenos Aires, under permit number 2014/01.
- Anonymous (2014) Boletín integrado de vigilancia. Ministerio de Salud de la República Argentina. Presidencia de la Nación. http://www.msal.gov.ar/images/stories/boletines/Boletin%20Integrado%20De%20Vigilancia%20N200-SE52.pdf. Accesed 11 June 2016
- Anonymous (2015a) Servicio Meteorológico Nacional. http://www.smn.gov.ar/. Accesed 11 June 2016
- Anonymous (2015b) Servicios Climáticos. Servicio Meteorológico Nacional. http://www.smn.gov.ar/serviciosclimaticos/clima/archivo/verano.pdf. Accesed 10 June 2016
- Fariña F, Scialfa E, Bolpe J, Pasqualetti M, Rosa A, Ribicich M (2012) Study of Trichinella spp. in rodents that live near pig farms in an endemic region of the province of Buenos Aires, Argentina. J Bacteriol Parasitol 3:140Google Scholar
- Gamble HR, Bessonov AS, Cuperlovic K, Gajadhar AA, Van Knapen F, Noeckler K, Schenone H, Zhu X (2000) International commission on trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild animals intended for human consumption. Vet Parasitol 93:393–408CrossRefPubMedGoogle Scholar
- Kapel CMO, Webster P, Lind P, Pozio E, Henriksen SA, Murrell KD, Nansen P (1998) Trichinella spiralis, Trichinella britovi, and Trichinella nativa: infectivity, larval distribution in muscle, and antibody response after experimental infection of pigs. Parasitol Res 84:264–271CrossRefPubMedGoogle Scholar