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Parasitology Research

, Volume 115, Issue 12, pp 4587–4593 | Cite as

Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique

  • N. K. Galaktionov
  • O. I. Podgornaya
  • P. P. Strelkov
  • K. V. Galaktionov
Original Paper

Abstract

The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability.

Keywords

AFLP H. elongata parthenitae Clonal diversity Monomiracidial infection 

Notes

Acknowledgments

This work was supported by the Russian Foundation for Basic Research (grants 09-04-01145-a and 16-04-00753-a) and Russian Scientific Fund (grant 15-15-20026). We deeply appreciate the constant support of the White Sea Biological Station of the Zoological Institute RAS Kartesh. We are grateful to the anonymous reviewers for their useful suggestions. The work was partially carried out at the Development of Molecular and Cellular Technologies Resource Center at the St. Petersburg State University.

Supplementary material

436_2016_5249_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 17 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • N. K. Galaktionov
    • 1
    • 2
  • O. I. Podgornaya
    • 2
    • 3
  • P. P. Strelkov
    • 1
  • K. V. Galaktionov
    • 1
    • 4
  1. 1.St. Petersburg State UniversitySt. PetersburgRussia
  2. 2.Institute of Cytology RASSt. PetersburgRussia
  3. 3.Far Eastern Federal UniversityVladivostokRussia
  4. 4.Zoological Institute RASSt. PetersburgRussia

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