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Parasitology Research

, Volume 115, Issue 10, pp 3991–3998 | Cite as

In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus

  • Fen Wang
  • Bin YeEmail author
Original Paper

Abstract

Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene of E. granulosus was in silico cloned. The B cell and T cell epitopes were predicted by bioinformatics methods. The cDNA sequence of EgTIM was composition of 1094 base pairs, with an open reading frame of 753 base pairs. The deduced amino acid sequences were composed of 250 amino acids. Five cross-reactive epitopes, locating on 21aa-35aa, 43aa-57aa, 94aa-107aa, 115-129aa, and 164aa-183aa, could be expected to serve as candidate epitopes in the development of vaccine against E. granulosus. These results could provide bases for gene cloning, recombinant expression, and the designation of anti-hydatidosis vaccine.

Keywords

Echinococcus granulosus Triosephosphate isomerase In silico cloning Bioinformatics Epitopes 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant numbers 30972567 and 30371258).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of Pathogenic BiologyChongqing Medical UniversityChongqingChina
  2. 2.Research Center for Molecule Medicine and TumorChongqing Medical UniversityChongqingChina

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