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Parasitology Research

, Volume 115, Issue 10, pp 3963–3972 | Cite as

Ultrastructural and molecular characterization of Glugea serranus n. sp., a microsporidian infecting the blacktail comber, Serranus atricauda (Teleostei: Serranidae), in the Madeira Archipelago (Portugal)

  • Graça Casal
  • Sónia Rocha
  • Graça Costa
  • Saleh Al-Quraishy
  • Carlos Azevedo
Original Paper

Abstract

A new microsporidian infecting the connective tissue of the coelomic cavity of the blacktail comber Serranus atricauda, in the Madeira Archipelago (Portugal), is described on the basis of morphological, ultrastructural, and molecular features. The microsporidian formed large whitish xenomas adhering to the peritoneal visceral organs of the host. Each xenoma consisted of a single hypertrophic cell, in the cytoplasm of which mature spores proliferated within parasitophorous vacuoles surrounded by numerous collagen fibers. Mature spores were ellipsoidal and uninucleated, measuring an average of 6.5 ± 0.5 μm in length and 3.4 ± 0.6 μm in width. The anchoring disk of the polar filament was subterminal, laterally shifted from the anterior pole of the spore. The isofilar polar filament coiled in 18–19 turns, forming two rows that surrounded the posterior vacuole. The latter occupied about one third of the spore length. The polaroplast surrounding the apical and uncoiled portion of the polar filament displayed two distinct regions: a lamellar region and an electron-dense globule. Molecular analysis of the rRNA genes, including the internal transcribed spacer region, and phylogenetic analysis using maximum likelihood and neighbor joining demonstrated that this microsporidian parasite clustered with some Glugea species. Based on the differences found both at the morphological and molecular levels, to other members of the genus Glugea, the microsporidian infecting the blacktail comber is considered a new species, thus named Glugea serranus n. sp.

Keywords

Fish parasite Microsporidia Fine structure rRNA genes 

Notes

Acknowledgments

This work was partially supported by Eng. António Almeida Foundation (Porto, Portugal); FCT (Lisbon, Portugal), within the scope of the PhD fellowship grant attributed to S. Rocha (SFRH/BD/92661/2013) through the program QREN-POPH/FSE; and the project no. PRG-1436-02 of King Saud University (Riyadh, Saudi Arabia). We are grateful to Dr. Paulo Oliveira, director of the Natural Park of Madeira, for permission to catch the samples of blacktail comber, from the coastal waters of the Selvagens Nature Reserve, and to the Nature Ranger, Mr. Jacques, for help with the line fishing. This work complies with the current laws of the country where it was performed. The helpful comments and suggestions of the reviewers were greatly appreciated.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Graça Casal
    • 1
    • 2
    • 3
  • Sónia Rocha
    • 2
    • 3
  • Graça Costa
    • 4
  • Saleh Al-Quraishy
    • 5
  • Carlos Azevedo
    • 2
    • 3
    • 5
  1. 1.Department of SciencesUniversity Institute of Health Sciences, CESPUGandraPortugal
  2. 2.Laboratory of Animal Pathology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/UP)University of PortoPortoPortugal
  3. 3.Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP)University of PortoPortoPortugal
  4. 4.Marine Biological Station of FunchalUniversity of MadeiraFunchalPortugal
  5. 5.Zoology Department, College of SciencesKing Saud UniversityRiyadhSaudi Arabia

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