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Parasitology Research

, Volume 115, Issue 10, pp 3795–3806 | Cite as

Schistosoma japonicum cystatin attenuates murine collagen-induced arthritis

  • Fang Liu
  • Weisheng Cheng
  • Faustina Pappoe
  • Xiaodong Hu
  • Huiqin Wen
  • Qingli Luo
  • Shushu Wang
  • Fang Deng
  • Yuanyuan Xie
  • Yuanhong Xu
  • Jilong Shen
Original Paper

Abstract

Recombinant SjCystatin (rSjCystatin), a recombinant protein of Schistosoma japonicum cystatin, has been reported to have an effect on immunoregulation mediated by IL-10 induction. Rheumatoid arthritis (RA) is a common autoimmune inflammatory arthropathy, and recombinant immune-modulating drugs for RA treatment are under development. We aimed to study the putative immune regulation of rSjCystatin and its prophylactic/therapeutic effects on murine collagen-induced arthritis (CIA). CIA was induced in DBA/1 mice by inoculation with bovine collagen II (CII). rSjCystatin was administered prior or post development of CIA. The severity of CIA was assessed using established clinical and histopathological scoring systems. The incidence was also determined. The CII-specific antibodies in sera and cytokines in splenocyte culture supernatants were measured by ELISA. Th1/Th2/Th17 cells and Tregs development in splenocytes were monitored by flow cytometry. The inflammatory mediators in the diseased joint were semiquantitated by qPCR. Prophylactic injection of rSjCystatin attenuated paw clinical scores, incidence, and histopathology scores of joints in CIA mice. The arthritis-alleviative effects were closely associated with the augmentation of IL-4, IL-10, and collagen-specific IgG1, and with the distinct reduction of IFN-γ, collagen-specific IgG2a, and the marked decrease of proinflammatory cytokines IL-6, IL-17, and TNF-α and RANKL. The data indicate that rSjCystatin may prevent cartilage destruction and inflammation of joints in CIA mice. The effects are related to the inhibitory modulation of Th1 and Th17 and upregulation of Tregs and Th2 via a shift of cytokines profiling from Th1 to Th2 response.

Keywords

Collagen-induced arthritis Schistosoma japonicum Cystatin Immune modulation 

Notes

Acknowledgments

The work was funded by the National Science Foundation of China (No. 81171606) and Science Foundation of Anhui Province (No. 1308085MH124). We would like to thank Li He from the Department of Human Parasitology, Basic Medical School, Tongji Medical College of HUST for the gift of recombinant plasmid of SjCystatin.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

436_2016_5140_MOESM1_ESM.doc (35 kb)
Table S1 Primers for qRT-PCR (DOC 35 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Fang Liu
    • 1
  • Weisheng Cheng
    • 1
    • 2
  • Faustina Pappoe
    • 1
  • Xiaodong Hu
    • 1
  • Huiqin Wen
    • 1
    • 3
  • Qingli Luo
    • 1
  • Shushu Wang
    • 1
  • Fang Deng
    • 4
  • Yuanyuan Xie
    • 1
  • Yuanhong Xu
    • 1
    • 2
    • 5
    • 6
  • Jilong Shen
    • 1
    • 2
    • 5
    • 6
  1. 1.Department of Pathogen Biology, Provincial Laboratory of Pathogen Biology and Key Laboratory of Zoonoses AnhuiAnhui Medical UniversityHefeiChina
  2. 2.Clinical Laboratorythe First Affiliated Hospital of Anhui Medical UniversityHefeiChina
  3. 3.Department of Blood Transfusionthe First Affiliated Hospital of Anhui Medical UniversityHefeiChina
  4. 4.Department of Laboratory Diagnosisthe Provincial Tumor Hospital of Anhui Medical UniversityHefeiChina
  5. 5.Department of Pathogen BiologyAnhui Medical UniversityHefeiChina
  6. 6.Department of Clinical Laboratory MedicineAnhui Medical UniversityHefeiChina

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