Advertisement

Parasitology Research

, Volume 115, Issue 9, pp 3599–3604 | Cite as

Henneguya melini n. sp. (Myxosporea: Myxobolidae), a parasite of Corydoras melini (Teleostei: Siluriformes) in the Amazon region: morphological and ultrastructural aspects

  • Patrick D. Mathews
  • Antônio A.M. Maia
  • Edson A. AdrianoEmail author
Original Paper

Abstract

A new species of myxozoan, Henneguya melini sp. n. (Myxosporea: Myxobolidae), was described based on morphologic and ultrastructural features. This is a parasite of the ornamental freshwater fish C. melini from the Rio Negro, and it was found in five of 30 (16.7 %) C. melini examined. The parasite was found in the gill filaments, and the plasmodia had form of round to ellipsoid, with mature and immature spores inside them. The average spore body was 15.5 ± 0.2 μm in length, 4.7 ± 0.1 μm in width, and the tail measured 25.3 ± 0.1 μm in length. The spores showed typical features of the genus Henneguya, with two valves of equal size and two symmetrical polar capsules of 4.8 ± 0.7 μm in length and 1.7 ± 0.3 μm in width. Each polar capsule had a polar filament with five to six turns. Based on morphology (morphologic and ultrastructural data) of the plasmodia and spores and the fact that this is the first report of a Henneguya species in a fish species of the genus Corydoras, it was considered a new myxozoan species.

Keywords

Myxozoa Henneguya Taxonomy Fish Amazon 

Notes

Acknowledgments

This study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (Proc. No. 2013/21374-6-Adriano E. A.). Mathews P.D. was supported by a doctoral scholarship from FAPESP (Proc. No. 2013/14656-5). Adriano E.A. received a research productivity grant from the Brazilian Fostering Agency CNPq (Proc. No. 305630/2013-0). CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) provides support to the Doctoral Program in Animal Biology of UNICAMP. The authors thank Dr. Geraldo Bernardino, secretary of Fishing of the Amazonas State, for help in the logistic of the field works and Dr. Christopher Berger for reviewing the language of the text.

References

  1. Abdallah VD, Azevedo RK, Luque JL, Bomfim TCB (2007) Two new species of Henneguya Thélohan, 1892 (Myxozoa, Myxobolidae), parasitic on the gills of Hoplosternum littorale (Callichthyidae) and Cyphocharax gilbert (Curimatidae) from the Guandu River, State of Rio de Janeiro, Brazil. Parasitol Latinoam 62:35–41CrossRefGoogle Scholar
  2. Abdel-Ghaffar F, Abdel-Baki AS, Bayoumy EM, Bashtar AR, Qurieshy SA, MorseyKS AA, Mehlhorn H (2008) Light and electron microscopic study on Henneguya suprabranchiae Landsberg, 1987 (Myxozoa: Myxosporea) infecting Oreochromis niloticus, a new host record. Parasitol Res 103:609–617CrossRefPubMedGoogle Scholar
  3. Adriano EA, Arana S, Cordeiro NS (2005) An ultrastructural and histopathological study of Henneguya pellucida n. sp. (Myxosporea: Myxobolidae) infecting Piaractus mesopotamicus (Characidae) cultivated in Brazil. Parasite 12(3):221–227CrossRefPubMedGoogle Scholar
  4. Adriano EA, Carriero MM, Maia AAM, Silva MRM, Naldoni J, Ceccarelli OS, Arana S (2012) Phylogenetic and host − parasite relationship analysis of Henneguya multiplasmodialis n. sp. infecting Pseudoplatystoma spp. in Brazilian Pantanal wetland. Vet Parasitol 185:110–120CrossRefPubMedGoogle Scholar
  5. Ali MA (1999) Henneguya ghaffari sp. n. (Myxozoa: Myxosporea), infecting the Nile perch Lates niloticus (Teleostei: Centropomidae). Dis Aquat Org 38:225–230CrossRefPubMedGoogle Scholar
  6. Ali MA, Abdel-Baki AS, Sakran TH, Entzeroth R, Abdel-Ghaffar F (2007) Myxobolus lubati n. sp. (Myxosporea: Myxobolidae), a new parasite of haffara seabream Rhabdosargus haffara (Forsskal, 1775), Red Sea, Egypt: a light and transmission electron microscopy. Parasitol Res 100:819–827CrossRefPubMedGoogle Scholar
  7. Azevedo C, Matos E (1995) Henneguya adherens n. sp. (Myxozoa, Myxosporea), parasite of the Amazon fish, Acestrorhynchus falcatus. J Eukaryot Microbiol 42:515–518CrossRefPubMedGoogle Scholar
  8. Barassa B, Adriano EA, Cordeiro NS, Ceccarelli PS (2012) Morphology and host-parasite interaction of Henneguya azevedoi n. sp., parasite of gills of Leporinus obtusidens from Mogi-Guaçu River, Brazil. Parasitol Res 110:887–894CrossRefPubMedGoogle Scholar
  9. Casal G, Matos E, Azevedo C (1997) Some ultrastructural aspects of Henneguya striolata sp. nov. (Myxozoa, Myxosporea), a parasite of the Amazonian fish Serrasalmus striolatus. Parasitol Res 83(1):93–95CrossRefPubMedGoogle Scholar
  10. Casal G, Matos E, Azevedo C (2003) Light and electron microscopic study of the myxosporean, Henneguya friderici n. sp. from the Amazonian teleostean fish, Leporinus friderici. Parasitology 126:313–319CrossRefPubMedGoogle Scholar
  11. Carriero MM, Adriano EA, Silva MRM, Ceccarelli PS, Maia AAM (2013) Molecular phylogeny of the Myxobolus and Henneguya genera with several new South American species. PLoS ONE 8:e73713CrossRefPubMedPubMedCentralGoogle Scholar
  12. Current WL, Janovy J Jr (1976) Ultrastructure of interlamellar Henneguya exilis in the channel catfish. J Parasitol 62:975–981CrossRefPubMedGoogle Scholar
  13. Dyková I, Lom J (1978) Histopathological changes in fish gills infected with myxosporidian parasites of the genus Henneguya. J Fish Biol 12:197–202CrossRefGoogle Scholar
  14. Eiras JC (2002) Synopsis of the species of the genus Henneguya Thelohan, 1892 (Myxozoa: Myxosporea: Myxobolidae. Syst Parasitol 52(1):43–54CrossRefPubMedGoogle Scholar
  15. Eiras JC, Takemoto RM, Pavanelli GC (2009) Henneguya corruscans n. sp. (Myxozoa, Myxosporea, Myxobolidae), a parasite of Pseudoplatystoma corruscans (Osteichthyes, Pimelodidae) from the Parana River, Brazil: a morphological and morphometric study. Vet Parasitol 159:154–158CrossRefPubMedGoogle Scholar
  16. Eiras JC, Adriano EA (2012) Checklist of the species of the genus Henneguya Thélohan, 1892 (Myxozoa, Myxosporea, Myxobolidae) described between 2002 and 2012. Syst Parasitol 83(2):95–104CrossRefPubMedGoogle Scholar
  17. El-Mansy AIE, Bashtar AR (2002) Histopathological and ultrastructural studies of Henneguya suprabranchiae Landsberg, 1987 (Myxosporea: Myxobolidae) parasitizing the suprabranchial organ of the freshwater catfish Clarias gariepinus Burchell, 1822 in Egypt. Parasitol Res 88:617–626CrossRefPubMedGoogle Scholar
  18. Feist SW, Longshaw M (2006) Phylum Myxozoa. In: Woo PTK (ed) Fish diseases and disorders: Protozoan and Metazoan infections, 2nd edn. CAB International, UK, pp 230–296CrossRefGoogle Scholar
  19. Feijó MM, Arana S, Ceccarelli PS, Adriano EA (2008) Light and scanning electron microscopy of Henneguya arapaima n. sp. (Myxozoa: Myxobolidae) and histology of infected sites in pirarucu (Arapaima gigas: Pisces: Arapaimidae) from the Araguaia River, Brazil. Vet Parasitol 157:59–64CrossRefPubMedGoogle Scholar
  20. Froese R, Pauly D (2011) FishBase. World Wide Web electronic publication. Version (02/2011) www.fishbase.org (accessed 10 May 2015)
  21. Griffin MJ, Pote LM, Wise DJ, Greenway TE, Mauel MJ, Camus AC (2008) A novel Henneguya species from channel catfish described by morphological, histological, and molecular characterization. J Aquat Anim Health 20:127–135CrossRefPubMedGoogle Scholar
  22. Hallett SL, Diamant A (2001) Ultrastructure and small-subunit ribosomal DNA sequence of Henneguya lesteri n. sp. (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Dis Aquat Org 46:197–212CrossRefPubMedGoogle Scholar
  23. Iwanowicz LR, Iwanowicz DD, Pote LM, Blazer VS, Schill WB (2008) Morphology and 18S rDNA of Henneguya gurlei (Myxosporea) from Ameiurus nebulosus (Siluriformes) in North Carolina. J Parasitol 94:46–57CrossRefPubMedGoogle Scholar
  24. Kostoïngue B, Diebakate C, Faye N, Toguebaye S (2001) Presence of Myxosporidea (Myxozoa: Myxosporea) of the genus Henneguya Thélohan, 1892 in freshwater fishes from Chad (Central Africa). Acta Protozool 40:117–123Google Scholar
  25. Landsberg JH (1987) Myxosporean parasites of the catfish, Clarias lazera (Valenciennes). Syst Parasitol 9:73–81CrossRefGoogle Scholar
  26. Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156CrossRefGoogle Scholar
  27. Martins ML, Onaka EM, Bozzo FR, Fenerick JJ (2004) Henneguya sp. (Myxozoa: Myxobolidae) in Pimelodus maculatus (Osteichthyes: Siluridae) from Volta Grande reservoir, Minas Gerais, Brazil. B Inst Pesca 30:1–7Google Scholar
  28. Matos E, Tajdari J, Azevedo C (2005) Ultrastructural studies of Henneguya rhamdia n. sp. (Myxozoa) a parasite from the Amazon teleost fish, Rhamdia quelen (Pimelodidae). J Eukaryot Microbiol 52:532–537CrossRefPubMedGoogle Scholar
  29. Mathews PD, Silva MRM, Maia AMA, Adriano EA (2015) Ultrastructure and ssrRNA sequencing of Myxidium amazonense n. sp. a myxosporean parasite of Corydoras melini from the Rio Negro river, Amazonas state, Brazil. Parasitol Res 114:4675–4683CrossRefPubMedGoogle Scholar
  30. Mathews PD, Maia AMA, Adriano EA (2016) Morphological and ultrastructural aspects of Myxobolus niger n. sp. (Myxozoa) gill parasite of Corydoras melini (Siluriformes: Callichthyidae) from Brazilian Amazon. Acta Trop 158:214–219CrossRefPubMedGoogle Scholar
  31. Mohammed AA, AL-Rasheid KA, Sakran T, Abdel-Baki AA, Abdel-Ghaffar FA (2002) Some species of the genus Myxobolus (Myxozoa: Myxosporea) infecting freshwater fish of the River Nile, Egypt, and the impact on their hosts. Parasitol Res 88:9–15Google Scholar
  32. Molnár K (1998) Taxonomic problems, seasonality and histopathology of Henneguya creplini (Myxosporea) infection of the pikeperch Stizostedion lucioperca in Lake Balaton. Folia Parasitol 45:261–269PubMedGoogle Scholar
  33. Molnár K, Székely C, Mohamed K, Shaharom-Harrison F (2006) Myxozoan pathogens in cultured Malaysian fishes. I. Myxozoan infections of the sutchi catfish Pangasius hypophthalmus in freshwater cage cultures. Dis Aquat Org 68:209–218CrossRefPubMedGoogle Scholar
  34. Morsy K, Abdel-Ghaffar F, Bashtar AR, Mehlhorn H, Al Quraishy S, Abdel-Gaber R (2012) Morphology and small subunit ribosomal DNA sequence of Henneguya suprabranchiae (Myxozoa), a parasite of the catfish Clarias gariepinus (Clariidae) from the River Nile, Egypt. Parasitol Res 111:1423–35CrossRefPubMedGoogle Scholar
  35. Naldoni J, Arana S, Maia AAM, Ceccarelli PS, Tavares LER, Borges FA, Pozo CF, Adriano EA (2009) Henneguya pseudoplatystoma n. sp. causing reduction in epithelial area of gills in the farmed pintado, a South American catfish: histopathology and ultrastructure. Vet Parasitol 166:52–59CrossRefPubMedGoogle Scholar
  36. Naldoni J, Arana S, Maia AAM, Silva MRM et al (2011) Host–parasite–environment relationship, morphology and molecular analysis of Henneguya eirasi n. sp. parasite of two wild Pseudoplatystoma ssp. in Pantanal Wetland, Brazil. Vet Parasitol 177:247–255CrossRefPubMedGoogle Scholar
  37. Naldoni J, Maia AAM, Da Silva MRM, Adriano EA (2014) Henneguya cuniculator sp. nov., a parasite of spotted sorubim Pseudoplatystoma corruscans in the São Francisco Basin, Brazil. Dis Aquat Org 107:211–221CrossRefPubMedGoogle Scholar
  38. Pote LM, Hanson LA, Shivaji R (2000) Small subunit ribosomal RNA sequences link the cause of proliferative gill disease in channel catfish to Henneguya n. sp. (Myxozoa: Myxosporea). J Aquat Anim Health 12:230–240CrossRefGoogle Scholar
  39. Rabie SA, Mohammed NI, Hussein AA, Hussein NM (2009) The infection of freshwater fishes with three species of Henneguya in Qena, Upper Egypt. Egypt Acad J Biol Sci 1:11–19Google Scholar
  40. Reed CC, Basson L, Van As LL (2003) Myxozoans infecting the sharptooth catfish, Clarias gariepinus in the Okavango River and Delta, Botswana, including descriptions of two new species, Henneguya samochimensis sp. n. and Myxobolus gariepinus sp. n. Folia Parasitol 50:183–189CrossRefPubMedGoogle Scholar
  41. Reis RE (1998) Systematic, Biogeography, and the fossil record of the Callichthyidae: a review of the available data. In: Malabarba LR, Reis RE, Vari RP, Lucena ZMS, Lucena CAS (eds) Phylogeny and Classification of Neotropical Fishes. Edipucrs, Porto Alegre, Brasil, pp 351–362Google Scholar
  42. Rocha E, Matos E, Azevedo C (1992) Henneguya amazonica n. sp. (Myxozoa, Myxobolidae), parasitizing the gills of Crenicichla lepidota Heckel, 1840 (Teleostei, Cichlidae) from Amazon River. Eur J Protistol 28(3):273–278CrossRefPubMedGoogle Scholar
  43. Sarkar NK (1985) Myxosporidan Henneguya mystusia sp. n. (Myxozoa: Myxosporea) from the gill of a freshwater teleost fish Mystus sp. Acta Protozool 241:55–58Google Scholar
  44. Sitjà-Bobadilla A (2008) Fish immune response to myxozoan parasites. Parasite 15:420–425CrossRefPubMedGoogle Scholar
  45. Videira M, Velasco M, Azevedo R, Silva R, Gonçalves E, Matos P, Matos E (2015) Morphological aspects of Henneguya aequidens n. sp. (Myxozoa: Myxobolidae) in Aequidens plagiozonatus Kullander, 1984 (Teleostei: Cichlidae) in the Amazon region, Brazil. Parasitol Res 114:1159–1162CrossRefPubMedGoogle Scholar
  46. Vita P, Corral L, Matos E, Azevedo C (2003) Ultrastructural aspects of the myxosporean Henneguya astyanax n. sp. (Myxozoa: Myxobolidae), a parasite of the Amazonian teleost Astyanax keithi (Characidae). Dis Aquat Org 53:55–60CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Patrick D. Mathews
    • 1
  • Antônio A.M. Maia
    • 2
  • Edson A. Adriano
    • 1
    • 3
    Email author
  1. 1.Departamento de Biologia Animal, Instituto de BiologiaUniversidade Estadual de Campinas (UNICAMP)CampinasBrazil
  2. 2.Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de AlimentosUniversidade de São Paulo (USP)PirassunungaBrazil
  3. 3.Departmento de Ciências BiológicasUniversidade Federal de São PauloDiademaBrazil

Personalised recommendations