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Molecular diversification of Trichuris spp. from Sigmodontinae (Cricetidae) rodents from Argentina based on mitochondrial DNA sequences

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Abstract

A molecular phylogenetic hypothesis is presented for the genus Trichuris based on sequence data from mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob). The taxa consisted of nine populations of whipworm from five species of Sigmodontinae rodents from Argentina. Bayesian Inference, Maximum Parsimony, and Maximum Likelihood methods were used to infer phylogenies for each gene separately but also for the combined mitochondrial data and the combined mitochondrial and nuclear dataset. Phylogenetic results based on cox1 and cob mitochondrial DNA (mtDNA) revealed three clades strongly resolved corresponding to three different species (Trichuris navonae, Trichuris bainae, and Trichuris pardinasi) showing phylogeographic variation, but relationships among Trichuris species were poorly resolved. Phylogenetic reconstruction based on concatenated sequences had greater phylogenetic resolution for delimiting species and populations intra-specific of Trichuris than those based on partitioned genes. Thus, populations of T. bainae and T. pardinasi could be affected by geographical factors and co-divergence parasite-host.

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Acknowledgments

We thank Carlos Galliari, Ulyses Pardiñas, Marcela Lareschi, Guillermo Panisse, Natalia Guerreiro Martins, and Juliana Notarnicola for their help during host collections; Carlos Galliari and Ulyses Pardiñas for the identification of the hosts; and Graciela T. Navone for her support in the laboratory. The present work was supported by a grant from the V Plan Propio de Investigación of the University of Sevilla, Spain, the Agencia Nacional de Promoción Científica y Tecnológica (PICT 2010–0924), and Proyecto de incentivos de la Universidad Nacional de La Plata (UNLP N 627 and travel grant).

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  1. Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Prof. García González 2, Sevilla, 41012, Spain

    Rocío Callejón & Cristina Cutillas

  2. Centro de Estudios Parasitológicos y de Vectores (CEPAVE), CCT–CONICET–La Plata/Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina

    María Del Rosario Robles

  3. Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina

    Carlos Javier Panei

  4. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    Carlos Javier Panei

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  1. Rocío Callejón
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Rocío Callejón and María Del Rosario Robles contributed equally to this paper and should be considered as co-first authors

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Callejón, R., Robles, M.D.R., Panei, C.J. et al. Molecular diversification of Trichuris spp. from Sigmodontinae (Cricetidae) rodents from Argentina based on mitochondrial DNA sequences. Parasitol Res 115, 2933–2945 (2016). https://doi.org/10.1007/s00436-016-5045-y

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