Detection of Balamuthia mandrillaris DNA in the storage case of contact lenses in Germany
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Acanthamoeba spp. are frequently the etiological agents of a severe form of sight-threatening keratitis, called Acanthamoeba keratitis. The contact lens storage solution of a patient with keratitis of unknown genesis was screened using our diagnostic tools to detect potentially pathogenic free-living amoebae (FLA). Culture methods and a triplex quantitative real-time polymerase chain reaction (qPCR) targeting Acanthamoeba spp., Naegleria fowleri, and Balamuthia mandrillaris were used in context of this routine screening. While no amoebae were detected by culture, qPCR specifically detected DNA of B. mandrillaris. This FLA is known as the etiological agent of a fatal form of encephalitis in humans and other mammals, Balamuthia amoebic encephalitis (BAE). A fragment of the 18S rDNA gene was amplified from the sample and showed 99 % sequence identity to B. mandrillaris sequences from GenBank. To the best of our knowledge, this is the first report of B. mandrillaris found in association with contact lenses. Although no viable amoeba was obtained by culturing efforts, the verification of B. mandrillaris DNA in the contact lens storage solution demonstrates how easily this pathogen might come into close contact with humans.
KeywordsBalamuthia mandrillaris Contact lense Quantitative real-time PCR
We thank Sebastian Njul for excellent technical assistance and David Lam (Shaman Medical Consulting) for review and English-language editing of the article.
- Adl SM, Simpson AG, Lane CE, Lukeš J, Bass D, Bowser SS, Brown MW, Burki F, Dunthorn M, Hampl V, Heiss A, Hoppenrath M, Lara E, Le Gall L, Lynn DH, McManus H, Mitchell EA, Mozley-Stanridge SE, Parfrey LW, Pawlowski J, Rueckert S, Shadwick RS, Schoch CL, Smirnov A, Spiegel FW (2012) The revised classification of eukaryotes. J Eukaryot Microbiol 59:429–493CrossRefPubMedPubMedCentralGoogle Scholar
- Cabello-Vílchez AM, Reyes-Batlle M, Montalbán-Sandoval E, Martín-Navarro CM, López-Arencibia A, Elias-Letts R, Guerra H, Gotuzzo E, Martínez-Carretero E, Piñero JE, Maciver SK, Valladares B, Lorenzo-Morales J (2014) The isolation of Balamuthia mandrillaris from environmental sources from Peru. Parasitol Res 113:2509–2513CrossRefPubMedGoogle Scholar
- Greninger AL, Messacar K, Dunnebacke T, Naccache SN, Federman S, Bouquet J, Mirsky D, Nomura Y, Yagi S, Glaser C, Vollmer M, Press CA, Klenschmidt-DeMasters BK, Dominguez SR, Chiu CY (2015) Clinical metagenomic identification of Balamuthia mandrillaris encephalitis and assembly of the draft genome: the continuing case for reference genome sequencing. Genome Med 7:113CrossRefPubMedPubMedCentralGoogle Scholar
- Nicholas K, Nicholas H, Deerfield D (1997) GeneDoc: analysis and visualization of genetic variation. Embnew News 4:14Google Scholar
- Page FC (1988) A new key to freshwater and soil Gymnamoebae with instructions for culture. Freshwater Biological Association, Ambleside, UKGoogle Scholar
- Retana-Moreira L, Abrahams-Sandí E, Cabello-Vílchez AM, Reyes-Batlle M, Valladares B, Martínez-Carretero E, Piñero JE, Lorenzo-Morales J (2014) Isolation and molecular characterization of Acanthamoeba and Balamuthia mandrillaris from combination shower units in Costa Rica. Parasitol Res 113:4117–4122CrossRefPubMedGoogle Scholar
- Roy SL, Atkins JT, Gennuso R, Kofos D, Sriram RR, Dorlo TP, Hayes T, Qvarnstrom Y, Kucerova Z, Guglielmo BJ, Visvesvara GS (2015) Assessment of blood–brain barrier penetration of miltefosine used to treat a fatal case of granulomatous amebic encephalitis possibly caused by an unusual Balamuthia mandrillaris strain. Parasitol Res 114:4431–4439CrossRefPubMedGoogle Scholar
- Schroeder JM, Booton GC, Hay J, Niszl IA, Seal DV, Markus MB, Fuerst PA, Byers TJ (2001) Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge. J Clin Microbiol 39:1903–1911CrossRefPubMedPubMedCentralGoogle Scholar