Parasitology Research

, Volume 115, Issue 5, pp 1965–1975 | Cite as

Evaluation of cellular retinoic acid binding protein 2 gene expression through the retinoic acid pathway by co-incubation of Blastocystis ST-1 with HT29 cells in vitro

  • Chen-Chieh Liao
  • Eing-Ju Song
  • Tsuey-Yu Chang
  • Wei-Chen Lin
  • Hsiao-Sheng Liu
  • Lih-Ren Chen
  • Lynn L. H. Huang
  • Jyh-wei Shin
Original Paper
First Online:
Received:
Accepted:

Abstract

Blastocystis is a parasitic protist with a worldwide distribution that is commonly found in patients with colon and gastrointestinal pathological symptoms. Blastocystis infection has also commonly been reported in colorectal cancer and HIV/AIDS patients with gastrointestinal symptoms. To understand the pathway networks of gene regulation and the probable mechanisms influencing functions of HT-29 host cells in response to parasite infection, we examined the expression of 163 human oncogenes and kinases in human colon adenocarcinoma HT-29 cells co-incubated with Blastocystis by in-house cDNA microarray and PCR analysis. At least 10 genes were shown to be modified following Blastocystis co-incubation, including those with immunological, tumorigenesis, and antitumorigenesis functions. The expression of genes encoding cellular retinoic acid binding protein 2 (CRABP2) and proliferating cell nuclear antigen (PCNA) was markedly upregulated and downregulated, respectively. Reverse transcriptase-PCR validated the modified transcript expression of CRABP2 and other associated genes such as retinoic acid (RA)-related nuclear-receptor (RARα). Together, our data indicate that CRABP2, RARα, and PCNA expressions are involved in RA signaling regulatory networks that affect the growth, proliferation, and inflammation of HT-29 cells.

Keywords

Blastocystis HT-29 colonic epithelial cell Cellular retinoic acid binding protein 2(CRABP2) Retinoic acid receptor (RAR) 
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Notes

Acknowledgments

Funding for this project was provided by the Ministry of Science and Technolog (MOST 104-2622-B-006-014) and the Ministry of Education, National Cheng Kung University.

Compliance with ethical standards

None

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Chen-Chieh Liao
    • 1
  • Eing-Ju Song
    • 2
  • Tsuey-Yu Chang
    • 3
  • Wei-Chen Lin
    • 3
  • Hsiao-Sheng Liu
    • 4
  • Lih-Ren Chen
    • 1
    • 5
  • Lynn L. H. Huang
    • 1
    • 6
    • 7
  • Jyh-wei Shin
    • 3
    • 4
  1. 1.Institute of Biotechnology, College of Bioscience and BiotechnologyNational Cheng Kung UniversityTainan CityTaiwan
  2. 2.Department of Bioscience Technology, College of Health SciencesChang Jung Christian UniversityTainan CityTaiwan
  3. 3.Department of Parasitology, Institute of Microbiology and Immunology, College of MedicineNational Cheng Kung UniversityTainan CityTaiwan
  4. 4.Institute of Medical Informatics, College of Electrical Engineering and Computer ScienceNational Cheng Kung UniversityTainan CityTaiwan
  5. 5.Livestock Research Institute, Council of AgricultureTainanTaiwan
  6. 6.Department of Biotechnology and Bioindustry Sciences, College of Bioscience and BiotechnologyNational Cheng Kung UniversityTainan CityTaiwan
  7. 7.Advanced Optoelectronic Technology CenterNational Cheng Kung UniversityTainan CityTaiwan

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