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Parasitology Research

, Volume 115, Issue 5, pp 1845–1851 | Cite as

Efficacy of oral afoxolaner plus milbemycin oxime chewables against induced infestations with Dermacentor reticulatus in dogs

  • Steffen RehbeinEmail author
  • Josephus J. Fourie
  • Christa de Vos
  • Andrew Anderson
  • Diane L. Larsen
  • Philippe Jeannin
Original Paper

Abstract

The efficacy of afoxolaner plus milbemycin oxime (AFX + MO) combination chewables (NexGard Spectra®, Merial) and AFX single-entity chewables (NexGard®, Merial) against induced infestations with Dermacentor reticulatus ticks was evaluated in dogs. Thirty dogs were assigned to blocks of three animals each based on pre-allocation tick counts and were randomly allocated to one of three groups: untreated (control), treated with a combination of AFX + MO chewables to be as close as possible to the minimum effective dose of AFX + MO (2.5 + 0.5 mg per kg body weight), and treated with a combination of NexGard® chewables to be as close as possible to the minimum effective dose of AFX (2.5 mg per kg body weight). Treatments were administered orally once on day 0. Starting 2 days before treatment administration, each dog was infested with approximately 50 ticks weekly for six consecutive weeks. Live ticks were counted at ∼48 h post-treatment (removal count) and at ∼48 h (in situ counts) and ∼72 h (removal counts) following each post-treatment infestation. Treatment with both AFX + MO and NexGard® chewables rapidly eliminated the existing tick infestations (100 % efficacy) within 2 days following treatment administration. Weekly re-infestations were controlled for a minimum of 5 weeks with the efficacy ranging from 92.2 to 99.7 % based on ∼48 h post-treatment in situ counts and between 99.0 and 100 % based on ∼72 h post-treatment removal counts (p < 0.0001 at each occasion). This study demonstrated a high efficacy of both AFX + MO chewable and NexGard® chewable treatments against infestations of dogs with D. reticulatus ticks for at least 5 weeks. In addition, this study indicated no interference between the two compounds with respect to the acaricidal activity provided by AFX.

Keywords

Dermacentor reticulatus Tick Afoxolaner Milbemycin oxime Dog 

Notes

Compliance of ethical standards

Conflict of interest

The work reported herein was funded by Merial, Inc., GA, USA. All authors are current/were employees or were contractors of Merial.

Disclaimer

This document is provided for scientific purposes only. Any reference to a brand or trademark herein is for informational purposes only and is not intended for a commercial purpose or to dilute the rights of the respective owner(s) of the brand(s) or trademark(s).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Steffen Rehbein
    • 1
    Email author
  • Josephus J. Fourie
    • 2
  • Christa de Vos
    • 2
  • Andrew Anderson
    • 3
  • Diane L. Larsen
    • 3
  • Philippe Jeannin
    • 4
  1. 1.Merial GmbH, Kathrinenhof Research CenterRohrdorfGermany
  2. 2.ClinVet International (Pty) Ltd.BloemfonteinRepublic of South Africa
  3. 3.Merial, Inc.DuluthUSA
  4. 4.Merial S.A.S., Centre de Recherche de Saint-VulbasSaint-VulbasFrance

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