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Parasitology Research

, Volume 115, Issue 4, pp 1659–1666 | Cite as

Identification of repellent odorants to the body louse, Pediculus humanus corporis, in clove essential oil

  • Takuma Iwamatsu
  • Daisuke Miyamoto
  • Hidefumi Mitsuno
  • Yoshiaki Yoshioka
  • Takeshi Fujii
  • Takeshi Sakurai
  • Yukio Ishikawa
  • Ryohei KanzakiEmail author
Original Paper

Abstract

The control of body lice is an important issue for human health and welfare because lice act as vectors of disease such as typhus, relapsing fever, and trench fever. Body lice exhibit avoidance behavior to some essential oils, including clove essential oil. Therefore, odorants containing clove essential oil components may potentially be useful in the development of repellents to body lice. However, such odorants that induce avoidance behavior in body lice have not yet been identified from clove essential oil. Here, we established an analysis method to evaluate the avoidance behavior of body lice to specific odorants. The behavioral analysis of the body lice in response to clove essential oil and its constituents revealed that eugenol, a major component of clove essential oil, has strong repellent effect on body lice, whereas the other components failed to induce obvious avoidance behavior. A comparison of the repellent effects of eugenol with those of other structurally related odorants revealed possible moieties that are important for the avoidance effects to body lice. The repellent effect of eugenol to body lice was enhanced by combining it with the other major component of clove essential oil, β-caryophyllene. We conclude that a synthetic blend of eugenol and β-caryophyllene is the most effective repellent to body lice. This finding will be valuable as the potential use of eugenol as body lice repellent.

Keywords

Body lice Behavior analysis Eugenol Synergistic effect 

Notes

Acknowledgments

This study was supported by Grant-in-Aid for JSPS Fellows.

Supplementary material

436_2016_4905_MOESM1_ESM.tif (84 kb)
ESM 1 GC-MS analysis of standard. a Total ion chromatogram of eugenol. b Mass spectrum of eugenol. Base ion and molecular ion, 164 (100 %); and diagnostic ion, 149 ([M?15]+, 37.5 %). (TIF 83.7 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Takuma Iwamatsu
    • 1
    • 2
  • Daisuke Miyamoto
    • 1
  • Hidefumi Mitsuno
    • 3
  • Yoshiaki Yoshioka
    • 4
  • Takeshi Fujii
    • 5
    • 6
  • Takeshi Sakurai
    • 3
  • Yukio Ishikawa
    • 5
  • Ryohei Kanzaki
    • 1
    • 3
    Email author
  1. 1.Department of Advanced Interdisciplinary Studies, Graduate School of EngineeringThe University of TokyoMeguro-kuJapan
  2. 2.Japan Society for the Promotion of Science (JSPS) Research Fellow, Kojimachi Business Center BuildingChiyodaJapan
  3. 3.Research Center for Advanced Science and TechnologyThe University of TokyoMeguro-kuJapan
  4. 4.Osaka Pharmaceutical Co., LtdHigashioosakaJapan
  5. 5.Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuJapan
  6. 6.Department of Biological Production, Faculty of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan

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