Parasitology Research

, Volume 115, Issue 3, pp 1349–1353 | Cite as

First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

  • Alireza Zahedi
  • Jordan Phasey
  • Tony Boland
  • Una Ryan
Short Communication


A molecular epidemiological survey of Cryptosporidium from water buffalo (Bubalus bubalis) in the Northern Territory in Australia was conducted. Fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18S quantitative PCR (qPCR). Positives were typed by sequence analysis of 18S nested PCR products. The qPCR prevalence of Cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. Sequence analysis identified two species: C. parvum and C. bovis, with C. parvum accounting for ~80 % of positives typed from the farmed buffalo fecal samples compared to 50 % for wild buffalo. Subtyping at the 60 kDa glycoprotein (gp60) locus identified C. parvum subtypes IIdA19G1 (n = 4) and IIdA15G1 (n = 1) in the farmed buffalo and IIaA18G3R1 (n = 2) in the wild buffalo. The presence of C. parvum, which commonly infects humans, suggests that water buffaloes may contribute to contamination of rivers and waterways with human infectious Cryptosporidium oocysts, and further research on the epidemiology of Cryptosporidium in buffalo populations in Australia is required.


Cryptosporidium Buffalo 18S C. parvum C. bovis gp60 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Alireza Zahedi
    • 1
  • Jordan Phasey
    • 2
  • Tony Boland
    • 3
  • Una Ryan
    • 1
  1. 1.School of Veterinary and Life SciencesMurdoch UniversityPerthAustralia
  2. 2.Indigenous Essential Services, Power and Water CorporationDarwinAustralia
  3. 3.Tropical Water Solutions Pty. Ltd.WoolnerAustralia

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