Advertisement

Parasitology Research

, Volume 115, Issue 3, pp 1203–1212 | Cite as

Larvicidal activity of catechin isolated from Leucas aspera against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae)

  • Devan Elumalai
  • Maduraiveeran Hemavathi
  • Periaswamy Hemalatha
  • Chandrasekar Vijayalakshmi Deepaa
  • Patheri Kunyil KaleenaEmail author
Original Paper
First Online:

Abstract

Vector control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of plant origin my serve as an alternative biocontrol technique in the future. The aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extracts of Leucas aspera on the fourth-instar larvae of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. The larvae were exposed to fractions with concentrations ranging from 1.25, 2.25, 5, 10, and 20 ppm and isolated compounds. After 24 h exposure, larval mortality was assessed. Among the eight fractions, four from hexane extractions showed potent larvicidal activity against tested mosquito species at 20 ppm concentration. The isolated compound catechin showed pronounced larvicidal activity at very low concentrations. The LC50 and LC90 values of catechin were 3.05 and 8.25 ppm against Ae. aegypti, 3.44 and 8.89 ppm against An. stephensi, and 3.76 and 9.79 ppm against C. quinquefasciatus, respectively. The isolated compound was subjected to spectral analyses (GC-MS, FTIR, 1H NMR, and 13C NMR) to elucidate the structure and to compare with spectral data literature.

Keywords

Leucas aspera Catechin Larvicidal activity Aedes aegypti Anopheles stephensi Culex quinquefasciatus 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

The authors are grateful to University Grants Commission (F.No.35-69/2008 (SR) dated 20 Mar 2009 for providing financial assistance to carry out the present investigations. Our thanks are also extended to the Principal, Presidency College, Chennai, for providing infrastructure and research facilities.

References

  1. Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267CrossRefGoogle Scholar
  2. Al-Mehmadi RM, Al-Khalaf AA (2010) Larvicidal and histological effects of Melia azedarach extract on Culex quinquefasciatus Say larvae (Diptera: Culicidae). J King Saud Univ 22:77–85CrossRefGoogle Scholar
  3. Bernhard L, Bernhard P, Magnussen P (2003) Management of Patient with lymphoiedema caused by filariasis in north eastern Tanzania: alternative approaches. Physiotherapy 89:743–749CrossRefGoogle Scholar
  4. Bernick EP, Moffet SB, Moffett DF (2007) Organization, ultrastructure and development of midgut visceral muscle in larval Aedes aegypti. Tiss Cell 39:277–292CrossRefGoogle Scholar
  5. Bond TJ, Lewis CJR, Davis AP, Davies C, Santos-Buelga G, Williamson E (2003) Methods in polyphenols analysis. Royal Society of Chemistry, Cambridge, UK, pp 229–266Google Scholar
  6. Boots AW, Haenen GR, Bast A (2008) Health effects of quercetin, from antioxidant to nutraceutical. Eur J Pharmacol 585:2–3CrossRefGoogle Scholar
  7. Chavsses DC, Yap HH (1997) Chemical methods for the control of vectors and pests of public health importance, Document WHO.97. 2 WHO. Geneva. Chemistry 115:650–656Google Scholar
  8. Chopra RN, Nair SL, Chopra IC (2002) Glossary of Indian medicinal plants. CSIR, New Delhi. 23Google Scholar
  9. David JP, Rey D, Pautou MP, Meyran JC (2000) Differential toxicity of leaf litter to dipteran larvae of mosquito developmental sites. J Invertebr Pathol 75:9–18CrossRefPubMedGoogle Scholar
  10. De Omena MC, DeNavarro DM, DePaula JE, Luna JS, Ferreira de Lima MR, Sant Ana AE (2007) Larvicidal activities against Aedes aegypti of some Brazilian medicinal plants. Bio Technol 98:2549–2556CrossRefGoogle Scholar
  11. Dean M (2001) Lymphatic filariasis: the quest to eliminate a 4000-year-old-disease. Hollis Publishing, New HampshireGoogle Scholar
  12. Donovan DL, Luthria P, Stremple AL, Waterhouse J (1999) Analysis of (1)-catechin, (2)-epicatechin and their 39- and 49-O-methylated analogs a comparison of sensitive methods. Chromatogr B 726:277–283CrossRefGoogle Scholar
  13. Dwivedi SC, Karwasara K (2003) Larvicidal activity of five plant extracts against Culex quinquefasciatus. Indian J Entomol 65:335–338Google Scholar
  14. Ghayal N, Padhye A, Dhumal K (2010) Larvicidal activity of invasive weeds Cassia uniflora and Synedrella nodiflora. Inter J Pharma Bio Sciences 1 (3)Google Scholar
  15. Green MM, Singer JM, Sutherland DJ, Hibben CR (1991) Larvicidal activity of Tagetes minuta (marigold) toward Aedes aegypti. J Am Mosq Contrl Assoc 7:282–286Google Scholar
  16. Hamouda LS, Elayassaki WM, Hamed MS (1996) Toxicity and histopathological effect of Artmisia judaic and Anagallis arvensis extracts on Culex pipiens larvae. J Egypt Ger Soc Zool 20:43–60Google Scholar
  17. ICMR Bulletin (2003) Prospects of using herbal products in the control of mosquito vectors. 33: 1–10Google Scholar
  18. Jang YS, Baek BR, Yang YC, Kim MK, Lee HS (2002) Larvicidal activity of leguminous seeds and grains against Aedes aegypti and Culex pipiens pallens. J Am Mosq Control Assoc 18:210–213PubMedGoogle Scholar
  19. Jin W, Tu PF (2005) Preparative isolation and purification of trans-3,5,4- trihydroxystilbene-4α-β-D-glucopyranoside and +) catechin from Rheum tanguticum Maxim. Ex Balf. using high-speed counter chromatography by stepwise elution and stepwise increasing the flow-rate of the mobile phase. J Chromatography A 1092:241–245CrossRefGoogle Scholar
  20. Joseph CC, Ndoile MM, Malima RC, Nkuniya MH (2004) Larvicidal and mosquitocidal extracts, a coumrin, isoflavonoids and pterocarpans from Neorautanenia mitis. T Roy Soc Trop Med H 98:451–455CrossRefGoogle Scholar
  21. Kaewnang OE, Ngampongsaim A, Subhadhirasakul S, Srichana T (2011) Toxicity of fixed oil and crude extract from sa-dao-thiam Azadirachta excelsa (Jack) seed kernel to Aedes aegypti (L.) Songklanakarin. J Sci Technol 33:43–49Google Scholar
  22. Kannathasan K, Senthilkumar A, Venkatesalu V (2011) Mosquito larvicidal activity of methyl-p-hydroxybenzoate isolated from the leaves of Vitex trifolia Linn. Acta Tropica 120:115–8CrossRefPubMedGoogle Scholar
  23. Kamalakannan S, Gobinath C, Ananth S (2014) Synthesis and characterization of fungus mediated silver nanoparticle for toxicity on filarial vector, Culex quinquefasciatus. Int J Pharm Sci Rev Res 24:124–132Google Scholar
  24. Kamaraj C, Rahuman AA, Bagavan A (2008) Antifeedant and larvicidal effect of plant extracts against Spodoptera litura (F), Aedes aegypti L. and Culex quinquefasciatus Say. Paras Rese 103:325–331CrossRefGoogle Scholar
  25. Karioti A, Bilia AR, Gabbiani C, Messori L, Skaltsa H (2009) Proanthocyanidin glycosides from the leaves of Quercus ilex L. (Fagaceae). Tetrahedron Lett 50:1771–1776Google Scholar
  26. Karunamoorthi K, Bekele M (2009) Prevalence of malaria from peripheral blood smears examination: a 1-year retrospective study from the Serbo Health Center, Kersa Woreda, Ethiopia. J Infect Public Health 2:171–176CrossRefPubMedGoogle Scholar
  27. Liu Y, Ye N, Liu R, Chen M, Zhang J (2010) H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. J Exper Botany 61:2979–2990CrossRefGoogle Scholar
  28. Maheswaran R, Kingsley S, Ignacimuthu S (2008) Larvicidal and repellent activity of Clerodendron phlomides against Culex quinquefasciatus Say (Diptera: Culicidae) proceed recent trends. Insect Pest Manage 240–243Google Scholar
  29. Mandal S (2011) Repellent activity of Eucalyptus and Azadirachta indica seed oil against the filarial mosquito Culex quinquefasciatus Say (Diptera: Culicidae) in India. Asian Pacific J Tropical Biomed 1(2):S109–S112CrossRefGoogle Scholar
  30. McGraw EA, ONeill SL (2013) Beyond insecticides: new thinking on an ancient problem. Nat Rev Microbiol 11:181–93CrossRefPubMedGoogle Scholar
  31. Morens DM, Fauci AS (2013) Emerging infectious diseases: threats to human health and global stability. PLoS Pathog 9(7):1003467CrossRefGoogle Scholar
  32. Perumalsamy H, Chang KS, Park C, Ahn YJ (2013) Larvicidal activity of Asarum heterotropoides root constituent’s against insecticide-susceptible and resistant Culex pipiens pallens and Aedes aegypti and Ochlerotatus togoi. J Agri Food Chem 58:10001–10006CrossRefGoogle Scholar
  33. Rajkumar G, Rahuman AA (2011) Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vectors. Acta Trop 118:196–203CrossRefGoogle Scholar
  34. Ray D, Pautou MP, Meyran JC (1999) Histopathological effects of tannic acid on the midgut epithelium of some aquatic diptera larvae. J Inver Patholog 73(2):173–181CrossRefGoogle Scholar
  35. Rocha AMCN, Morais AMMB (2001) Characterization of polyphenoloxidase (PPO) extracted from Jonagored apple. Food Control 12:85–90CrossRefGoogle Scholar
  36. Sharma P, Mohan L, Srivastava CN (2005) Larvicidal potential of Nerium indicm and Thiya orientelis extracts against malaria and Japanese encephalitis vector. J Envi Biol 26:67–70Google Scholar
  37. Shimomura H, Sashida Y, Yoshinari K (1989) Phenolic glucosides from the heartwood of Prunus grayana. Phytochemistry 28:1499–1502Google Scholar
  38. Suman TY, Elumalai D, Vignesh A, Kaleena PK, Murugesan K (2012) Evaluation of larvicidal activity of the aerial extracts of a medicinal plant, Ammannia baccifera (Linn) against two important species of mosquitoes, Aedes aegypti and Culex quinquefasciatus. Asian Pac J Trop Dis 352:355Google Scholar
  39. World Health Organization (2005) Communicable disease tool kit. World Health Organization, WHO/CDS/2005.26, Sudan, pp 68–72Google Scholar
  40. Yan G, Li S, Hu J, Zhai X, Ma W, Li N (2004) Phenolic constituents from the roots of Rosa laevigata (Rosaceae). Bioch System Ecology 52:23–26CrossRefGoogle Scholar
  41. Zhang M, Ying BP, Kubo I (1992) Nagilactones from Podocarpus nagi and their effects on the feeding and growth of tobacco budworm. J Nat Prod 55:1057–1062CrossRefGoogle Scholar
  42. Zhang WJ, Liu YQ, Li XC, Yang CR (1995) Chemical constituents of ecological tea from Yunnan. Acta Botanica Yunnanica 17:204–208Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Devan Elumalai
    • 1
  • Maduraiveeran Hemavathi
    • 2
  • Periaswamy Hemalatha
    • 1
  • Chandrasekar Vijayalakshmi Deepaa
    • 3
  • Patheri Kunyil Kaleena
    • 1
    Email author
  1. 1.Department of ZoologyPresidency College (Autonomous)ChennaiIndia
  2. 2.Department of ZoologyUniversity of MadrasChennaiIndia
  3. 3.Department of ChemistryPresidency College (Autonomous)ChennaiIndia

Personalised recommendations

Cite article

Buy options