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Parasitology Research

, Volume 115, Issue 2, pp 751–759 | Cite as

Biosynthesis, characterization, and acute toxicity of Berberis tinctoria-fabricated silver nanoparticles against the Asian tiger mosquito, Aedes albopictus, and the mosquito predators Toxorhynchites splendens and Mesocyclops thermocyclopoides

  • Palanisamy Mahesh Kumar
  • Kadarkarai Murugan
  • Pari Madhiyazhagan
  • Kalimuthu Kovendan
  • Duraisamy Amerasan
  • Balamurugan Chandramohan
  • Devakumar Dinesh
  • Udaiyan Suresh
  • Marcello Nicoletti
  • Mohamad Saleh Alsalhi
  • Sandhanasamy Devanesan
  • Hui Wei
  • Kandasamy Kalimuthu
  • Jiang-Shiou Hwang
  • Annalisa Lo Iacono
  • Giovanni BenelliEmail author
Original Paper

Abstract

Aedes albopictus is an important arbovirus vector, including dengue. Currently, there is no specific treatment for dengue. Its prevention solely depends on effective vector control measures. In this study, silver nanoparticles (AgNPs) were biosynthesized using a cheap leaf extract of Berberis tinctoria as reducing and stabilizing agent and tested against Ae. albopictus and two mosquito natural enemies. AgNPs were characterized by using UV–vis spectrophotometry, X-ray diffraction, and scanning electron microscopy. In laboratory conditions, the toxicity of AgNPs was evaluated on larvae and pupae of Ae. albopictus. Suitability Index/Predator Safety Factor was assessed on Toxorhynchites splendens and Mesocyclops thermocyclopoides. The leaf extract of B. tinctoria was toxic against larval instars (I–IV) and pupae of Ae. albopictus; LC50 was 182.72 ppm (I instar), 230.99 ppm (II), 269.65 ppm (III), 321.75 ppm (IV), and 359.71 ppm (pupa). B. tinctoria-synthesized AgNPs were highly effective, with LC50 of 4.97 ppm (I instar), 5.97 ppm (II), 7.60 ppm (III), 9.65 ppm (IV), and 14.87 ppm (pupa). Both the leaf extract and AgNPs showed reduced toxicity against the mosquito natural enemies M. thermocyclopoides and T. splendens. Overall, this study firstly shed light on effectiveness of B. tinctoria-synthesized AgNPs as an eco-friendly nanopesticide, highlighting the concrete possibility to employ this newer and safer tool in arbovirus vector control programs.

Keywords

Arbovirus Copepods Culicidae Dengue Predaceous mosquito larvae Predatory safety factor SEM Suitability index XRD 

Notes

Acknowledgments

The authors would like to thank the financial support rendered by King Saud University, through Vice Deanship of Research Chairs. The authors are grateful to the UGC-MRP, New Delhi, India (No. F. No.36-250/2008 (SR) 24/03/2009) and the Department of Physics and Astronomy, King Saud University (project no. RGP-1435- 057) for their financial support.

Compliance with ethical standards

All applicable international and national guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Conflict of interest

The authors declare no conflicts of interest. G. Benelli is an Editorial Board Member of Parasitology Research. This does not alter the author’s adherence to all the Parasitology Research policies on sharing data and materials.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Palanisamy Mahesh Kumar
    • 1
  • Kadarkarai Murugan
    • 1
  • Pari Madhiyazhagan
    • 1
  • Kalimuthu Kovendan
    • 1
  • Duraisamy Amerasan
    • 1
  • Balamurugan Chandramohan
    • 1
  • Devakumar Dinesh
    • 1
  • Udaiyan Suresh
    • 1
  • Marcello Nicoletti
    • 2
  • Mohamad Saleh Alsalhi
    • 3
  • Sandhanasamy Devanesan
    • 3
  • Hui Wei
    • 4
  • Kandasamy Kalimuthu
    • 1
    • 5
  • Jiang-Shiou Hwang
    • 5
  • Annalisa Lo Iacono
    • 6
  • Giovanni Benelli
    • 7
    Email author
  1. 1.Division of Entomology, Department of Zoology, School of Life SciencesBharathiar UniversityCoimbatoreIndia
  2. 2.Department of Environmental BiologyUniversity Sapienza of RomeRomeItaly
  3. 3.Department of Physics and Astronomy, Research Chair in Laser Diagnosis of CancerKing Saud UniversityRiyadhKingdom of Saudi Arabia
  4. 4.Institute of Plant ProtectionFujian Academy of Agricultural SciencesFuzhouChina
  5. 5.Institute of Marine BiologyNational Taiwan Ocean UniversityKeelungTaiwan
  6. 6.AgronomistPisaItaly
  7. 7.Department of Agriculture, Food and EnvironmentUniversity of PisaPisaItaly

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