Screening and characterization of early diagnostic antigens in excretory–secretory proteins from Trichinella spiralis intestinal infective larvae by immunoproteomics
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The excretory–secretory (ES) antigens from Trichinella spiralis muscle larvae are the most commonly used diagnostic antigens for trichinellosis, but specific IgG antibodies were not detected in early stage of infection. The aim of this study was to identify early diagnostic antigens from ES proteins of intestinal infective larvae (IIL), the first invasive stage of T. spiralis. Six bands (92, 52, 45, 35, 32, and 29 kDa) of IIL ES proteins were recognized by infection sera in Western blotting as early as 10 days post infection. Total of 54 T. spiralis proteins in six bands were identified by shotgun LC-MS/MS, 30 proteins were annotated, and 27 had hydrolase activity. Several proteins (serine protease, putative trypsin, deoxyribonuclease II family protein, etc.) could be considered as the potential early diagnostic antigens for trichinellosis. Our study provides new insights for screening early diagnostic antigens from intestinal worms of T. spiralis.
KeywordsTrichinella spiralis Intestinal infective L1 larvae (IIL1) Immunoproteomics Diagnostic antigens
The authors wish to thank Shanghai Applied Protein Technology Co. Ltd. for the technology support. This work was supported by National Natural Science Foundation of China (nos. 81471981, 81271860).
Conflict of interest
All authors declare that they have no competing interests.
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