Parasitology Research

, Volume 115, Issue 1, pp 217–223

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

Original Paper

Abstract

This study was conducted to estimate the prevalence of Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections among stray and pet cats in Lanzhou, northwest China, and to identify the influence of age, gender, and regions on seropositivity. T. gondii antibodies were examined in cat sera by the modified agglutination test (MAT). The circulating antigens of D. immitis and FeLV and specific antibodies to FIV were examined using kits commercially available. The overall prevalence of T. gondii, FIV, FeLV, and D. immitis was 19.34, 9.12, 11.33, and 3.04 %, respectively. For the genetic characterization of T. gondii genotypes in cats, genomic DNA was extracted from the seropositive cats and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genotyped using multilocus PCR-RFLP. Two T. gondii genotypes (ToxoDB#9 and ToxoDB#1) were identified. Results of the multivariate logistic regression analysis showed that older cats are more likely to be seropositive than juveniles for T. gondii, FIV, FeLV, and D. immitis. This is the first report of T. gondii genotypes in cats in northwest China. Moreover, the present study is the first study of retrovirus and D. immitis seroprevalence in cats in China. The results revealed that T. gondii, FIV, and FeLV infections are common in stray and pet cats in northwest China.

Keywords

Toxoplasma gondii Dirofilaria immitis Feline immunodeficiency virus Feline leukemia virus Cats Northwest China 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyJilin Agricultural UniversityChangchunPeople’s Republic of China
  2. 2.State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouPeople’s Republic of China
  3. 3.Jilin Entry-Exit Inspection and Quarantine BureauChangchunPeople’s Republic of China
  4. 4.Unité d’Epidémiologie, Laboratoire de Santé AnimaleANSESMaisons-AlfortFrance
  5. 5.Laboratoire de Parasitologie, EA3800, IFR53, CHU Reims, Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC), Reims, USC EpitoxoAnses LerpazFrance
  6. 6.Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary MedicineYangzhou UniversityYangzhouPeople’s Republic of China

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