Parasitology Research

, Volume 114, Issue 11, pp 4259–4266 | Cite as

Molecular characterization of Stictodora tridactyla (Trematoda: Heterophyidae) from Kuwait Bay using rDNA ITS and mtCO1

  • Wafa Y. Al-Kandari
  • Majed A. Alnaqeeb
  • Asha M. Isaac
  • Suzanne A. Al-Bustan
Original Paper
First Online:
Received:
Accepted:

Abstract

Stictodora tridactyla is an intestinal fluke in the family Heterophyidae that parasitizes shorebirds and mammals, including humans. Its metacercarial cyst stage was reported in the Arabian killifish, Aphanius dispar, at Kuwait Bay. In the present study, Cerithidea cingulata was found to serve as the first intermediate host of S. tridactyla. In order to establish the snail-fish link in the life cycle of S. tridactyla, complete sequences of ribosomal DNA internal transcribed spacer region 1 and 2 (rDNA ITS1 and ITS2) and partial sequence of cytochrome oxidase subunit 1 were obtained for metacercarial cysts isolated from the fish A. dispar and rediae isolated from the snail C. cingulata. Sequence alignment demonstrated that these larval stages belong to the same heterophyid species, S. tridactyla. Phylogenetic analysis based on rDNA ITS1, ITS2, and mtCO1 confirmed the position of S. tridactyla within the Heterophyidae and found it to cluster with Haplorchis spp. The present study represents the first molecular study correlating the larval stages of S. tridactyla using rDNA ITS1, ITS2, and mtCO1 and examining the phylogenetic relationships of S. tridactyla with different heterophyid species.

Keywords

Heterophyidae Stictodora tridactyla Cerithidea cingulata ITS1 ITS2 mtCO1 
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Notes

Acknowledgments

This work was supported by Kuwait University Research Grant No. SL07/10. Molecular analysis was performed with the support of the General Facility Project (GS 01/02) in the use of the ABI 3130xl Gene Analyzer at the Biotechnology Center, Faculty of Science, Kuwait University.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Wafa Y. Al-Kandari
    • 1
  • Majed A. Alnaqeeb
    • 1
  • Asha M. Isaac
    • 1
  • Suzanne A. Al-Bustan
    • 1
  1. 1.Department of Biological Sciences, Faculty of ScienceKuwait UniversitySafatKuwait

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