Parasitology Research

, Volume 114, Issue 10, pp 3747–3757

Ultrastructural characters of the spermatozoa in Digeneans of the genus Bianium Stunkard, 1930 (Digenea, Lepocreadiidae) parasites of fishes: a comparative study of Bianium plicitum and Bianium arabicum

  • Yann Quilichini
  • Papa Ibnou Ndiaye
  • Aminata Sène
  • Jean-Lou Justine
  • Rodney A. Bray
  • Vasyl V. Tkach
  • Cheikh Tidiane Bâ
  • Bernard Marchand
Original Paper

Abstract

We present in this paper the first ultrastructural study of the spermatozoon of species of the genus Bianium Stunkard, 1930, namely Bianium plicitum and Bianium arabicum belonging to the family Lepocreadiidae. Specimens are parasites of the tetraodontid teleosts Lagocephalus laevigatus caught in the Atlantic Ocean and Lagocephalus sceleratus caught in Pacific Ocean, respectively. Live worms were fixed and processed for transmission electron microscopy. Observations of numerous cross and longitudinal sections of the spermatozoa of B. plicitum and B. arabicum demonstrated the general pattern described in all previously studied Lepocreadioidea, namely two axonemes with 9+ “1” pattern of Trepaxonemata, two mitochondria, a nucleus, parallel cortical microtubules, and an external ornamentation of the plasma membrane associated with spine-like bodies. The maximum number of cortical microtubules and the external ornamentation were not located at the anterior extremity of the spermatozoon but at a more posterior level, as in all the species of Lepocreadioidea studied to date. Differences observed between spermatozoa of B. plicitum and B. arabicum were observed in: (1) the location of the first mitochondrion, (2) size of cortical microtubules which were longer in B. arabicum than in B. plicitum, and (3) the order of the disappearance of the axonemes, mitochondrion, and cortical microtubules toward the posterior region of the spermatozoon. Only the nucleus reaches into the posterior extremity of the spermatozoon in these two species. The general pattern of the spermatozoon described in these two species exhibit the general model in the genus Bianium.

Keywords

Spermatozoon Bianium spp. Digenea Ultrastructure Electron microscopy 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yann Quilichini
    • 1
  • Papa Ibnou Ndiaye
    • 2
  • Aminata Sène
    • 2
  • Jean-Lou Justine
    • 3
  • Rodney A. Bray
    • 4
  • Vasyl V. Tkach
    • 5
  • Cheikh Tidiane Bâ
    • 2
  • Bernard Marchand
    • 1
  1. 1.SERME Service d’Etude et de Recherche en Microscopie ElectroniqueUMR 6134-SPE, CNRS – Università di CorsicaCorteFrance
  2. 2.Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and TechniquesCheikh Anta Diop University of DakarDakarSenegal
  3. 3.Institut de Systématique, Évolution, Biodiversité, ISYEB, UMR7205 CNRS, EPHE, MNHN, UPMC, Muséum National d’Histoire NaturelleSorbonne UniversitésParisFrance
  4. 4.Department of Life SciencesNatural History MuseumLondonUnited Kingdom
  5. 5.Department of BiologyUniversity of North DakotaGrand ForksUSA

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