Completion of the life cycle of Tylodelphys mashonense (Sudarikov, 1971) (Digenea: Diplostomidae) with DNA barcodes and rDNA sequences
- 194 Downloads
The life cycle of Tylodelphys mashonense (Digenea: Diplostomidae), whose metacercariae occur in the cranial cavity of the widely cultivated catfish Clarias gariepinus, was resolved by the application of molecular markers. Both COI barcodes and ITS sequences obtained from diplostomid-like cercariae infecting Bulinus spp. from Mindu Dam, Morogoro, matched those acquired from metacercariae from the catfish C. gariepinus, and those from adult T. mashonense from the grey heron Ardea cinerea and the white egret Egretta alba. The success in linking the life cycle stages of T. mashonense using molecular tools highlights the usefulness of this approach in resolving the complex life cycles of digeneans in the absence of experimental establishment.
KeywordsLife cycle Tylodelphys mashonense Clarias gariepinus Ardea cinerea Egretta alba Bulinus spp.
This work was supported financially by the World Bank CIBI through the Faculty of Science, University of Dares Salaam, under the umbrella of the Science, Technology and Higher Education Project (STHEP), coordinated and implemented by the Ministry of Education and Vocational Training and the Ministry of Communication’s Science and Technology (MCST), a Natural Sciences and Engineering Research Council of Canada Discovery Grant A6979 to J. D. McLaughlin, and funding from the Canadian Genomics Research and Development Initiative to D. J. Marcogliese. We are also indebted to the Tanzania Fisheries Research Institute (TAFIRI) for granting us permission to use their laboratories. We thank Angela Rose Lapierre, who provided indispensable technical assistance, and an anonymous reviewer for helpful critical comments.
- Barson M, Rodney B, Ollevier F, Huyse T (2008) Taxonomy and faunistics of the helminth parasites of Clarias gariepinus (Burchell, 1822), and Oreochromis mossambicus (Peters, 1852) from temporary pans and pools in the Save-Runde River floodplain, Zimbabwe. Comp Parasitol 75:228–240CrossRefGoogle Scholar
- Beverley-Burton M (1963) A new strigeid Diplostomum (T) mashonensen.sp (Trematoda: Diplostomatidae) from the Grey Heron, Ardea Cinerea L., in Southern Rhodesia with an experimental demonstration of part of the life cycle. Rev Zool Bot Afr LXVIII:291–306Google Scholar
- Brown DS (2005) Freshwater snails of Africa and their medical importance, 2nd edn. Taylor & Fransis, LondonGoogle Scholar
- Chappell LH, Hardie LJ, Secombes CJ (1994) Diplostomiasis: the disease and host-parasite interactions. In: Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara Publishing Limited, Dyefed, pp 59–86Google Scholar
- Chibwana FD, Blasco-Costa I, Georgieva S, Hosea KM, Nkwengulila G, Scholz T, Kostadinova A (2013) A first insight into the barcodes for African diplostomids (Digenea: Diplostomidae): brain parasites in Clarias gariepinus (Siluriformes: Clariidae). Infect Genet Evol 17:62–70CrossRefPubMedGoogle Scholar
- Cribb TH, Andersona GR, Adlard RD, Bray RA (1998) A DNA-based demonstration of a three-host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). Int J Parasitol 28:680–684Google Scholar
- De Graaf G, Janssen H (1996) Artificial reproduction and pond rearing of the Arican catfish Clarias gariepinus in sub-Saharan Africa—a handbook. Food and Agricultural Organization of the United Nations, RomeGoogle Scholar
- El-Naffar MK (1979) Parasites of the Egyptian moorhens. I: Diplostomum magnicaudumsp. nov. with part of its life cycle. J Egypt Soc Parasitol 9:349–358Google Scholar
- El-Naffar MK, Khalifa RM, Sakla AA (1980) Parasitofauna of the Egyptian aquatic birds. II. Trematode parasites of the Giant Heron (Ardea goliath) in Assiut Governorate. J Egypt Soc Parasitol 10:107–116Google Scholar
- Faltýnková A (2005) Larval trematodes (Digenea) in molluscs from small water bodies near Šeské Budšjovice, Czech Republic. Acta Parasitol 50:49–55Google Scholar
- Kristensen TK (1987) A field guide to African freshwater snails. II. East African Species. Danish Bilharziasis Laboratory, CharlottenlundGoogle Scholar
- Locke SA, Mclaughlin JD, Marcogliese DJ (2010b) DNA barcodes show cryptic diversity and a potential physiological basis for host specificity among Diplostomoidea (Platyhelminthes: Digenea) parasitizing freshwater fishes in the St. Lawrence River, Canada. Mol Ecol 19:2813–2827CrossRefPubMedGoogle Scholar
- Musiba MJ, Nkwengulila G (2006) Occurrence of metacercariae of Diplostomum and Tylodelphys species (Diplostomidae) in Clarias species (Clariidae) from Lake Victoria. Tan J Sci 32:89–98Google Scholar
- Mwita C, Nkwengulila G (2004) Parasites of Clarias gariepinus (Butchell, 1822) from the Mwanza Gulf, Lake Victoria. Tan J Sci 30:53–62Google Scholar
- Niewiadomska K (1996) The genus Diplostomum—taxonomy, morphology and biology. Acta Parasitol 41:55–66Google Scholar
- Nkwengulila G, Kigadye ESP (2005) Occurrence of digenean larvae in freshwater snails in the Ruvu Basin, Tanzania. Tan J Sci 31:23–30Google Scholar
- Overstreet RM, Curran SS, Pote LM, King DT, Blend CK, Grater WD (2002) Bolbophorus damnificus n. sp. (Digenea: Bolbophoridae) from the channel catfish Ictalurus punctatus and American white pelican Pelecanus erythrorhynchos in the USA based on life-cycle and molecular data. Syst Parasitol 52:81–96CrossRefPubMedGoogle Scholar
- Quaggiotto EA, Valverde F (1994) Larval stages of digenetic trematodes in Chilina sp. (Mollusca, Pulmonata) of some water bodies of Patagonia, Argentina. Bol Chil Parasitol 50:33–36Google Scholar