Parasitology Research

, Volume 114, Issue 10, pp 3675–3682 | Cite as

Completion of the life cycle of Tylodelphys mashonense (Sudarikov, 1971) (Digenea: Diplostomidae) with DNA barcodes and rDNA sequences

  • F. D. Chibwana
  • G. Nkwengulila
  • S. A. Locke
  • J. D. McLaughlin
  • D. J. Marcogliese
Original Paper

Abstract

The life cycle of Tylodelphys mashonense (Digenea: Diplostomidae), whose metacercariae occur in the cranial cavity of the widely cultivated catfish Clarias gariepinus, was resolved by the application of molecular markers. Both COI barcodes and ITS sequences obtained from diplostomid-like cercariae infecting Bulinus spp. from Mindu Dam, Morogoro, matched those acquired from metacercariae from the catfish C. gariepinus, and those from adult T. mashonense from the grey heron Ardea cinerea and the white egret Egretta alba. The success in linking the life cycle stages of T. mashonense using molecular tools highlights the usefulness of this approach in resolving the complex life cycles of digeneans in the absence of experimental establishment.

Keywords

Life cycle Tylodelphys mashonense Clarias gariepinus Ardea cinerea Egretta alba Bulinus spp. 

Notes

Acknowledgments

This work was supported financially by the World Bank CIBI through the Faculty of Science, University of Dares Salaam, under the umbrella of the Science, Technology and Higher Education Project (STHEP), coordinated and implemented by the Ministry of Education and Vocational Training and the Ministry of Communication’s Science and Technology (MCST), a Natural Sciences and Engineering Research Council of Canada Discovery Grant A6979 to J. D. McLaughlin, and funding from the Canadian Genomics Research and Development Initiative to D. J. Marcogliese. We are also indebted to the Tanzania Fisheries Research Institute (TAFIRI) for granting us permission to use their laboratories. We thank Angela Rose Lapierre, who provided indispensable technical assistance, and an anonymous reviewer for helpful critical comments.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • F. D. Chibwana
    • 1
  • G. Nkwengulila
    • 1
  • S. A. Locke
    • 2
  • J. D. McLaughlin
    • 3
  • D. J. Marcogliese
    • 4
  1. 1.Department of Zoology and Wildlife EcologyUniversity of Dares SalaamDares SalaamTanzania
  2. 2.Department of BiologyUniversity of Puerto RicoMayagüezUSA
  3. 3.Department of BiologyConcordia UniversityMontrealCanada
  4. 4.Aquatic Contaminants Research Division, Water Science and Technology Directorate, Science and Technology BranchEnvironment CanadaMontrealCanada

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